Career Center

The Research Assistant is a member of the Cell and Developmental Biology Department within Basic Sciences at Vanderbilt University and is a key individual in the CeNGEN project to map neuron-specific gene expression in the model organism, C. elegans and in research that uses molecular genetics and live cell imaging to link neuron-specific gene expression to synapse formation. Reporting to the Principal Investigator, the Research Assistant interacts with students, staff, and faculty within the laboratory.

This position will provide an opportunity to use Fluorescence Activated Cell Sorting (FACS) to isolate specific neurons from the model organism, C. elegans, for RNA-Seq analysis in the CeNGEN project. The RA will use molecular genetics and live cell imaging to investigate synapse formation. Other tasks: Operate fluorescence microscopes to monitor GFP expression in nematode cultures. Maintain transgenic nematode strains and databases. Assist with daily operation of the laboratory (e.g. ordering supplies, maintaining equipment, assist undergraduate students in media preparation, plate pouring, etc.).

The Department of Cell and Developmental Biology is a vibrant, interdisciplinary environment for cutting-edge research over a scale that spans seven powers of ten, from single molecules to whole organisms. The difference between a test tube and a cell is spatio-temporal organization, and we study molecular, cellular and tissue organization in many of the laboratories within our department, seeking insights into fundamental biological questions and human disease. This year we achieved the #3 ranking in the nation for funding, as compared with similar departments in research institutions and universities. We are an interactive and highly collaborative department, with a strong graduate student association, outstanding core facilities, a top-ranked developmental biology program, and exceptionally strong faculty. Recent faculty recruits are studying cell migration using super-resolution microscopy, single molecule analysis of microtubule dynamics, systems biology of intestinal epithelia, stem cell biology, and the regulation of plasma membrane composition and aging.

Duties and Responsibilities

• Learn to use Fluorescence Activated Cell Sorting (FACS) to isolate C. elegans cells for RNA-sequencing for the CeNGEN project
• Use molecular genetics and live cell imaging to investigate the formation of electrical synapses in the nervous system of C. elegans.
• Works with Laboratory Manager to maintain freezers, prepare competent cells, ship nematode strains and reagents to other laboratories, order supplies and maintain equipment.
• Prepares sterile media, pour nutrient agar plates, inoculate bacterial cultures.

Qualifications

• A Bachelor's degree in Biology or a related field from an accredited institution of higher education is necessary.
• Experience with cell culture is preferred.
• Experience with recombinant DNA techniques is preferred.
• Experience with genome sequence data bases is preferred.
• Experience with C. elegans is preferred.
• Experience with preparing sterile growth media and agar plates is preferred.
• Ability to maintain quality, safety, and/or infection control standards is preferred.
• Ability to collect and process research data and accurately record findings is preferred.
• Basic knowledge and understanding of scientific research methodology is preferred.
• Ability to analyze and interpret statistical data is preferred.
• Working knowledge of research techniques, instrumentation, and/or equipment, as specified in the research study is preferred.
• Knowledge of data management techniques is preferred.

Commitment to Equity, Diversity, and Inclusion

At Vanderbilt University, we are intentional about and assume accountability for fostering advancement and respect for equity, diversity, and inclusion for all students, faculty, and staff. Our commitment to diversity makes us who we are. We have created a community that celebrates differences and lets individuality thrive. As part of this commitment, we actively value diversity in our workplace and learning environments as we seek to take advantage of the rich backgrounds and abilities of everyone. The diverse voices of Vanderbilt represent an invaluable resource for the University in its efforts to fulfill its mission and strive to be an example of excellence in higher education.

Vanderbilt University is an equal opportunity, affirmative action employer. Women, minorities, people with disabilities and protected veterans are encouraged to apply.

Please note, all candidates selected for an offer of employment are subject to pre-employment background checks, which may include but are not limited to, based on the role for which they have been selected: criminal history, education verification, social media review, motor vehicle records, credit history, and professional license verification.