The Department of Genetic Medicine is seeing a Research Technologist responsible for sample handling, planning and executing molecular genetics tests (i.e., genotyping or sequencing) on large numbers of DNA samples.
Specific Duties & Responsibilities
Barcode and enter sample associated information into the database.
Transfer DNA samples into 96-well plate format.
Receive and prepare Deepwell DNA stock plates and create working DNA plates.
Determine concentration and volume of DNA samples using various instruments.
Dilute samples to working concentrations.
Maintain and monitor inventory of all in-house DNA samples and reagent supplies.
Create master mixes; Set up polymerase chain reactions; trouble-shoot such reactions.
Operate and troubleshoot robotic liquid handlers.
Prepare and complete SNP genotyping assays including, but not limited to, Illumina assays and workflows.
Prepare samples for targeted capture of specific regions of DNA for sequencing experiments. This includes library preparation and capture techniques.
Prepare and complete Next-Gen sequencing assays including, but not limited to, Illumina Nova Seq workflows.
Perform quality control checks of data using various software, including, but not limited to, Genome Studio, Spotfire and other in-house QC metrics reports.
Maintain timely and accurate laboratory records (electronic and written).
Collect, organize, interpret and submit experimental data to database and supervisors.
Perform general lab maintenance duties.
Perform validation tests of lab reagents.
Participate in committees that function to improve processes.
Communicate effectively to supervisor and team members.
Perform miscellaneous related duties as assigned.
Special Knowledge, Skills, & Abilities
Basic understanding of molecular biology and genetics.
Ability to safely operate scientific laboratory equipment.
Able to work flexible hours beginning as early as 6:00 a.m. and/or ending as late as 6:00 p.m.
Superior organizational skills for time and data management.
Ability to function well in a team production setting.
Centrifuges, Illumina BeadLab system (Tecans, iScans, TeFlow), Covaris, BioAnalyzer, LabChip GX Touch, Qubit, Nanodrop instruments, Illumina NovaSeq, MiSeq and iSeq, thermal cyclers, robotic and manual liquid handlers, common laboratory equipment, Macintosh, PC computers, UNIX servers, Oracle database.
Sitting in a normal seated position for extended periods of time.
Reaching by extending hand(s) or arm(s) in any direction.
Finger dexterity to manipulate objects with fingers rather than with whole hand or arm, for example, using a keyboard.
Communication skills using the spoken word and ability to see and hear within normal parameters.
Ability to move about workspace and lift, carry, move supplies, computers, etc.
Bachelor's Degree in Biology, Chemistry or related field.
Some related post-undergraduate work experience preferred.
Classified Title: Research Technologist Role/Level/Range: ACRO40/E/02/CC Starting Salary Range: Min $15.50 - Max $26.00 HRLY ($43,160 targeted; Commensurate with experience) Employee group: Full Time Schedule: M-F Flexible Schedule 6AM-6PM Exempt Status: Non-Exempt Location: School of Medicine Campus Department name: SOM Genetics Ctr for Inhrtd Dis Res CIDR Personnel area: School of Medicine
Total Rewards The referenced salary range is based on Johns Hopkins University's good faith belief at the time of posting. Actual compensation may vary based on factors such as geographic location, work experience, market conditions, education/training and skill level. Johns Hopkins offers a total rewards package that supports our employees' health, life, career and retirement. More information can be found here: https://hr.jhu.edu/benefits-worklife/
Please refer to the job description above to see which forms of equivalency are permitted for this position. If permitted, equivalencies will follow these guidelines: JHU Equivalency Formula: 30 undergraduate degree credits (semester hours) or 18 graduate degree credits may substitute for one year of experience. Additional related experience may substitute for required education on the same basis. For jobs where equivalency is permitted, up to two years of non-related college course work may be applied towards the total minimum education/experience required for the respective job.
**Applicants who do not meet the posted requirements but are completing their final academic semester/quarter will be considered eligible for employment and may be asked to provide additional information confirming their academic completion date.
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