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Posted: 22-Nov-25
Location: St. Petersburg, Florida
Internal Number: 119490-en_US 1
We are seeking a Research Specialist II to join the lab at Johns Hopkins All Children's Hospital in St. Petersburg, Florida. This position will work in a lab focused on cancer immunology research investigating the tumor microenvironment and anti-tumor immune responses, including organoid and primary cell culture systems and in vivo preclinical tumor models. The specialist will contribute to immunotherapy and translational research studies within a collaborative and dynamic laboratory environment.
The Research Specialist II develops assays and protocols for PI approval and designs experimental approaches for laboratory projects. Performs a range of sophisticated assays. Makes significant contributions to various aspects of laboratory efforts and goals. Responsible for troubleshooting, independent decisions and training/guidance of other laboratory personnel.
Specific Duties & Responsibilities
- Conduct assigned laboratory projects.
- Utilize specialized knowledge to interpret results, e.g. read controls, evaluate results, etc.
- Work independently and use scientific knowledge and lab experience to make judgments and decisions.
- Teach/train learners, and new technicians in the laboratory.
- Guide, direct, and facilitate problem solving for entry-level laboratory members on day-to-day problems with more challenging research assays.
- Make scientific contributions to protocol development, efficiency, and productivity.
- Collaborate in the development of new techniques and instruments.
- Apply published research methods to current experimental work by investigating published protocols and discussing them with PI.
- Scientifically contribute to the preparation of manuscripts and proposals.
- Process analyzed data into figures for scientific publications.
- Order equipment and supplies, interface with vendors.
- Maintain daily records of experiments.
- Organize and track laboratory samples.
- Other duties as assigned.
Minimum Qualifications
- Bachelor's Degree in Biology, Chemistry or related field
- Two years of laboratory experience performing relevant techniques including experimental techniques used in genomics, sequencing, and cancer biology assays.
- Additional education may substitute for required experience and additional related experience may substitute for required education beyond a high school diploma/graduation equivalent, to the extent permitted by the JHU equivalency formula.
Preferred Qualifications
- Master's Degree with related graduate research.
- Cell culture.
- RNA isolation and quantitative PCR (qPCR).
- Common cell biology assays.
- Genomics and sequencing.
- Immunostaining.
- Western blotting.
- FACS analysis.
- Mouse handling.
- Bioinformatics analysis.
- GraphPad/Prism/Adobe.
Technical Skills & Expected Level of Proficiency
- Assay Development and Validation - Developing
- Data Management and Analysis - Developing
- Experimental Design and Execution - Developing
- Operations Management - Developing
- Protocol Development and Optimization - Developing
- Scientific Writing - Developing
- Stakeholder Engagement - Developing
The core technical skills listed are most essential; additional technical skills may be required based on specific division or department needs
Classified Title: Research Specialist II
Role/Level/Range: ACRP/03/MB
Starting Salary Range: $41,300 - $72,300 Annually (Commensurate w/exp.)
Employee group: Full Time
Schedule: Mon-Fri, 8.30am-5.00pm
FLSA Status: Exempt
Location: Florida
Department name: All Childrens
Personnel area: School of Medicine