The Division of Cardiology is seeking a Research Specialist II for a laboratory investigating the role of candidate gene mutations in thrombosis and hemostasis. Under the general supervision of a faculty investigator the Research Specialist II will independently perform routine and esoteric assays in the investigation of the effect of various genetic mutations on thrombosis. The person in this position will be expected to organize and present data to the faculty investigator. Responsibilities also include contributing to the general maintenance of the laboratory, equipment, and supplies.

Specific Duties and Responsibilities

• Follow established procedures and utilize appropriate technique to perform experiments associated with investigating the role of candidate gene mutations in thrombosis and hemostasis.
• Perform gene expression assays and assist in molecular cloning experiments.
• Maintain human endothelial and hepatocyte cell lines and execute cell-based assays and experiments.
• Perform standard protein assays to determine the effects of experimental manipulations on target protein levels.
• Assist in small animal model husbandry and experiments.
• Be familiar with basic laboratory techniques such as pipetting, buffer and reagent preparation, pH determination, qPCR, RT-qPCR, western blotting, ELISA spectrophotometric absorbance determination and fluorescence detection.
• Operate basic laboratory equipment such as centrifuge, pH meter, analytical balance, scintillation counter, light and/or fluorescence microscope, microplate reader, plate washer and biological safety cabinet.
• May also utilize sophisticated instrumentation, spectrophotometer, thermal cycler, flow cytometer, and laboratory automation solutions/robotics.
• Responsible for operation of lab computers for data entry.
• Maintain accurate data records and laboratory notebook.
• Maintain and make necessary adjustments to equipment, e.g. calibrating, alignment.
• Prepare solutions, reagents, and stains following standard laboratory formulas and procedures.
• Along with the laboratory supervisor, develop and institute standard operating procedures.
• Demonstrate proficiency in both written and verbal scientific communication.

• Bachelor's Degree in Biology, Chemistry or related field.
• Two years laboratory experience performing relevant techniques.
• Proficiency in standard biochemistry and cellular and molecular biology experimental techniques.

• Additional education may substitute for required experience and additional related experience may substitute for required education beyond HS Diploma/Graduation Equivalent, to the extent permitted by the JHU equivalency formula.

• Prior small animal research experience

Classified Title: Research Specialist II 
Role/Level/Range: ACRP/03/MB  
Starting Salary Range: $41,300 - $72,300 Annually ($42,000 targeted; Commensurate w/exp.) 
Employee group: Full Time 
Schedule: M-F, 9:30 AM - 6:00 PM 
FLSA Status: Exempt 
Location: School of Medicine Campus  
Department name: SOM DOM Cardiology   
Personnel area: School of Medicine 

About Johns Hopkins University

Johns Hopkins University remains committed to its founding principle, that education for all students should be grounded in exploration and discovery. Hopkins students are challenged not just to learn but also to advance learning itself. Critical thinking, problem solving, creativity, and entrepreneurship are all encouraged and nourished in this unique educational environment. After more than 130 years, Johns Hopkins remains a world leader in both teaching and research. Faculty members and their research colleagues at the university's Applied Physics Laboratory have each year since 1979 won Johns Hopkins more federal research and development funding than any other university. The university has nine academic divisions and campuses throughout the Baltimore-Washington area. The Krieger School of Arts and Sciences, the Whiting School of Engineering, the School of Education and the Carey Business School are based at the Homewood campus in northern Baltimore. The schools of Medicine, Public Health, and Nursing share a campus in east Baltimore with The Johns Hopkins Hospital. The Peabody Institute, a leading professional school of music, is located on Mount Vernon Place in downtown Bal...timore. The Paul H. Nitze School of Advanced International Studies is located in Washington's Dupont Circle area.

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