We are seeking a driven Research Specialist to join a laboratory that uses mouse models of bone marrow transplantation (BMT) to study the epigenetic mechanisms contributing to the development of graft-versus-host disease (GVHD) and the graft-versus-leukemia (GVL) effect.

Specific Duties and Responsibilities

• Conduct murine BMT experiments using established protocols that include,

                -  Harvest of murine spleen for single cell suspension

                - Splenic T-cell purification

                - Splenic T-cell flow cytometry

                - Harvest of murine bone marrow

                - Animal irradiation

                - Tail vein injections

• Participate in long-term monitoring of experiments, including examination of animals and GVHD scoring using an established clinical scale.
• Assist with analyzing and interpreting murine GVHD and survival data.
• Participate in post-transplant analyses, including the procurement of organs for fixation, and single-cell suspensions for surface and intracellular multiparameter flow cytometry staining of lymphoid and myeloid cells.
• Analyze and interpret flow cytometry data.
• Collaborate with the PI to oversee scientific management of the laboratory.
• Collaborate with PI or other lab members on research projects
• Identify and select the best options for scientific strategies and research development based on literature reviews and collaboration with colleagues.
• Perform a range of laboratory assays. Contribute to writing of scientific publications and grant applications.
• Act as a resource for laboratory techniques for post-doctoral fellow and students and junior technicians.

• Bachelor's Degree in Biology, Chemistry or related field.
• One year laboratory experience performing relevant techniques.
• Additional education may substitute for required experience and additional related experience may substitute for required education beyond High School Diploma or graduation equivalent, to the extent permitted by the JHU equivalency formula.

Classified Title: Research Specialist 
Job Posting Title (Working Title): Research Specialist   
Role/Level/Range: ACRP/03/MA  
Starting Salary Range: $17.11 - $29.90 HRLY ($23.51/hour targeted; Commensurate w/exp.) 
Employee group: Full Time 
Schedule: Mon - Fri / 8am - 4:30p 
FLSA Status: Non-Exempt 
Location: School of Medicine - East Baltimore Campus 
Department name: 10002936-SOM Onc Pediatric Oncology 
Personnel area: School of Medicine 

About Johns Hopkins University

Johns Hopkins University remains committed to its founding principle, that education for all students should be grounded in exploration and discovery. Hopkins students are challenged not just to learn but also to advance learning itself. Critical thinking, problem solving, creativity, and entrepreneurship are all encouraged and nourished in this unique educational environment. After more than 130 years, Johns Hopkins remains a world leader in both teaching and research. Faculty members and their research colleagues at the university's Applied Physics Laboratory have each year since 1979 won Johns Hopkins more federal research and development funding than any other university. The university has nine academic divisions and campuses throughout the Baltimore-Washington area. The Krieger School of Arts and Sciences, the Whiting School of Engineering, the School of Education and the Carey Business School are based at the Homewood campus in northern Baltimore. The schools of Medicine, Public Health, and Nursing share a campus in east Baltimore with The Johns Hopkins Hospital. The Peabody Institute, a leading professional school of music, is located on Mount Vernon Place in downtown Bal...timore. The Paul H. Nitze School of Advanced International Studies is located in Washington's Dupont Circle area.

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