The Department of Neurology is seeking a Research Technologist to work in a molecular virology lab. In this molecular virology (HIV-1, biosafety level 2) laboratory the candidate will utilize their solid foundation in basic laboratory techniques and experience with molecular biology and primary cell culture which requires a high level of rigor and reproducibility. Capable of exercising independent judgment while participating in the planning of experiments and interpreting results. The projects require care in data collection, preparation of reports and interfacing with sponsors. Works effectively as a team-member and exhibits excellent interpersonal, organizational, and self-management skills. Will provide training to other individuals who may be assigned to the laboratory on a regular or temporary basis.

Specific Duties & Responsibilities 

• Mouse breeding and husbandry.
• Handling and performing blood draw and injections on mice infected with viral pathogens.
• Isolation of peripheral blood lymphocytes and monocytes from human blood.
• Perform in vitro infection studies with HIV-1.
• Performs general molecular biology techniques such as cloning, qPCR, and Western blotting.
• Performs specialized techniques including multicomponent single-cell labeling, confocal and high-content microscopy, and multi-color flow cytometry.
• Experience and or interest in conducting single-cell bioinformatics is desirable.

Additional Knowledge, Skills and Abilities

• Familiarity with isolation of PBMCs and culture of primary human cells; sterile technique.
• Must be willing to work under Biohazard Level 2 Safety conditions with HIV-1.
• Must display willingness to learn and implement new technologies.
• Ability for self-management, integrity and professionalism.

• Bachelor's Degree in Biology, Chemistry or related field.
• Occasional irregular work hours consistent with research needs related to the in vivo studies with mice is required.
• Additional education may substitute for required experience and additional related experience may substitute for required education beyond HS Diploma/Graduation Equivalent, to the extent permitted by the JHU equivalency formula.

• Some related post-undergraduate work experience preferred

Classified Title: Research Technologist  
Role/Level/Range: ACRO40/E/02/CC  
Starting Salary Range: $15.70 - $26.25 HRLY (Commensurate w/exp.) 
Employee group: Full Time 
Schedule: M-F 8:30 am - 5:00 pm 
FLSA Status: Non-Exempt 
Location: School of Medicine Campus 
Department name: SOM Neuro Neuroimmunology  
Personnel area: School of Medicine 

About Johns Hopkins University

Johns Hopkins University remains committed to its founding principle, that education for all students should be grounded in exploration and discovery. Hopkins students are challenged not just to learn but also to advance learning itself. Critical thinking, problem solving, creativity, and entrepreneurship are all encouraged and nourished in this unique educational environment. After more than 130 years, Johns Hopkins remains a world leader in both teaching and research. Faculty members and their research colleagues at the university's Applied Physics Laboratory have each year since 1979 won Johns Hopkins more federal research and development funding than any other university. The university has nine academic divisions and campuses throughout the Baltimore-Washington area. The Krieger School of Arts and Sciences, the Whiting School of Engineering, the School of Education and the Carey Business School are based at the Homewood campus in northern Baltimore. The schools of Medicine, Public Health, and Nursing share a campus in east Baltimore with The Johns Hopkins Hospital. The Peabody Institute, a leading professional school of music, is located on Mount Vernon Place in downtown Bal...timore. The Paul H. Nitze School of Advanced International Studies is located in Washington's Dupont Circle area.

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