Position Summary:

• 10X single-cell RNA sequencing : a. Perform quality checks (QC30 value), determine the cut-off value for rarely expressed genes, and perform FDR-analysis. b. Knowledge of script language (Python, R, Linux, etc.) that enables the development of pipelines for individual projects. c. Understand and interpret borderline QC data. d. Familiarity with scripts that allow for the visual representation of data, including but not limited to river plots, clonal analysis, Partek analysis, dendrograms, heat maps, circos, etc. e. TCR/BCR sequencing, including the determination of clonality.
• 10X Visium HD spatial Transcriptomics: a. Runs sequencing alignments with single-cell images using cloud-based or LINUX platforms. b. Generates and prepares data in an easy-to-understand format, such as functional and phenotypic characteristics of cells present in the tissue section.
• Molecular Responsiveness testing (MRT- Molecular responsiveness testing for Cell Therapy-clinical samples). Develops, tests, and runs new pipelines on existing NCBI or other databases to detect clonal expansion, single nucleotide polymorphisms, mutations, and HLA-type and analyze tumor cell antigen presentation, T cell function, apoptosis, and inflammatory pathway. The Bioinformatics Specialist should also be efficient in the graphical presentation of data and perform statistical analysis of data, mainly multivariate analysis.
• Ncounter Nanostring assays Understands the sufficiency of reads obtained, the false discovery rate, and how to avoid it in analysis and data presentation. Generates graphical data sets and perform data comparisons among groups.
• Metabolomics: a. Understands metabolic pathways, biochemistry fundamentals, and how metabolites are generated and processed in human body. b. Knowledge of databases that store metabolite information (e.g., HMDB, KEGG) and experience integrating metabolomics with other omics data. c. Maintains familiarity with software and tools like MetaboAnalyst and others for analyzing metabolomics datasets, including targeted and untargeted metabolomics. d. Works with pathway enrichment analysis tools like KEGG Mapper, or IPA for identifying pathways associated with metabolomic signatures. e. Maintains strong understanding of statistical methods for high-dimensional data, including multivariate analysis linear models, and familiarity with machine learning approaches (supervised and unsupervised) that are often used in metabolomics for biomarker discovery and classification f. Familiarity with techniques for adjusting for potential confounders in metabolomics studies (e.g., host factors, batch effects).

Minimum Education and Experience:

• Master's Degree in Biology, Computational Biology and/or Computer Science or other related field.
• A minimum of two (2) years' work/research experience in bioinformatics, computer science, biology, biochemistry or similar.
• Equivalent education, experience and/or training may be substituted for the degree requirement.

City: New Brunswick

State: NJ

Physical Demands and Work Environment:

• Standing, sitting, walking, talking and hearing.
• No special vision requirements.
• Lifting up to 25 lbs.

• Flexible Work Arrangement possible per Rutgers University policies.
• Office environment. Moderate noise.

Posting Number: 25ST0860

About Rutgers University

Rutgers, The State University of New Jersey, is a leading national public research university and the state's preeminent, comprehensive public institution of higher education. Rutgers is dedicated to teaching that meets the highest standards of excellence; to conducting research that breaks new ground; and to turning knowledge into solutions for local, national, and global communities. As it was at our founding in 1766, the heart of our mission is preparing students to become productive members of society and good citizens of the world. Rutgers teaches across the full educational spectrum: preschool to precollege; undergraduate to graduate and postdoctoral; and continuing education for professional and personal advancement. Rutgers is New Jersey's land-grant institution and one of the nation's foremost research universities, and as such, we educate, make discoveries, serve as an engine of economic growth, and generate ideas for improving people's lives.

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