The UCSF Division of Cardiology/Cardiovascular Research Institute (CVRI) is seeking a highly motivated and skilled full-time Histology Technician to support cutting-edge research on cardiovascular biology using primarily mouse models (embryonic and adult). The successful candidate will play a critical role in the preparation, analysis, and imaging of tissue samples, contributing to groundbreaking discoveries in the field.
Key Responsibilities:
* Tissue Preparation and Sectioning:
o Perform fixation, processing, and embedding of mouse tissues (both embryonic and adult) for histological analysis.
o Section tissues using microtomy (paraffin) and cryotomy (frozen) techniques with high precision.
* Histological and Molecular Staining:
o Execute standard histological stains (e.g., H&E, Masson's trichrome).
o Conduct specialized staining protocols, including immunohistochemistry (IHC), immunofluorescence (IF), and advanced RNA in situ hybridization techniques such as RNAscope.
o Optimize and troubleshoot staining protocols as required.
* Microscopy and Structural Identification:
o Utilize light, fluorescent, and confocal microscopy to analyze stained tissue samples.
o Identify and document key structures of interest, ensuring accuracy and relevance to specific research objectives.
o Collaborate with researchers to confirm findings and provide interpretative input when necessary.
* Spatial Transcriptomics:
o Gain and apply expertise in spatial transcriptomic technologies to map gene expression within tissue samples.
o Prepare and process tissue for spatial transcriptomic experiments, adhering to rigorous quality control standards.
o Collaborate with bioinformatics teams to integrate spatial data into broader research contexts.
* Data Documentation and Quality Control:
o Maintain accurate and detailed records of all sample preparation, staining protocols, and analytical results.
o Ensure consistency and reproducibility of histological processes by adhering to established standard operating procedures (SOPs).
o Participate in the evaluation and implementation of new technologies and methods to enhance histological capabilities.
* Lab Collaboration and Communication:
o Work closely with a multidisciplinary team of researchers, including molecular biologists, physiologists, and bioinformaticians.
o Provide technical support and training to team members as needed.
o Attend and contribute to lab meetings, presenting data and updates on histological findings.
Required qualifications:
* Bachelor's degree in biology, biochemistry, molecular biology, or a related science field OR at least four years of research experience.
* Hands-on experience in tissue preparation, sectioning, and a variety of staining techniques (e.g., RNAscope, IHC, IF).
* Proficiency with microscopy (light, fluorescence, confocal) and identifying tissue structures.
* Familiarity with or willingness to learn spatial transcriptomic techniques.
* Strong attention to detail and excellent organizational skills.
* Ability to work both independently and collaboratively in a dynamic research environment.
Preferred qualifications:
* Experience with mouse models and tissue handling.
* Knowledge of cardiovascular biology or related research fields.
* Competence in troubleshooting and optimizing histological protocols.
Please apply online at https://aprecruit.ucsf.edu/JPF05443. Applicants' materials must list current and/or pending qualifications upon submission.
See Table 24B for the salary range for this position. A reasonable estimate for this position is $53,100-$56,600.
