We are seeking a Research Technologist to work under the general supervision of a faculty investigator.  Will be responsible for performing routine analyses and tests on animal or human fluids and/or tissue or cultured cell lines. The Hahn lab focuses on novel epigenetic diagnostic and therapeutic approaches to improve outcomes of patients and families affected by bladder cancer. Over the course of the summer, the research technologists will be introduced to basic laboratory techniques (cell culture, MTT assessment, DNA/RNA extraction, qPCR, Western blot, etc.) commonly used in medical research labs throughout the world. The applicant will be taught how to perform some siRNA experiments in bladder cancer cell lines in which they will knock down expression of specific zinc finger transcripts that have been identified as epigenetically silenced in human bladder cancer from data generated from the TCGA bladder cancer project. The research technologist will study the effects of knocking down these zinc finger transcripts on cell proliferation, invasion, and gene expression as well as response to therapy.

Specific Duties and Responsibilities

• Work requires basic use of scientific methods, procedures and techniques gained through previous training and experience.
• Uses basic understanding of theory behind experiments and applies basic analytical skills to implement assigned projects, and to identify and trouble-shoot situations needing special attention.
• Follows established procedures or protocols and utilizes aseptic technique to perform laboratory tests and/or experiments which may include basic tissue culture, cell separation methods, electrophoretic separations, assays, basic molecular biology, or other specialized histology and biological science techniques. May utilize in vivo models in experiments.
• Monitors inventory levels, orders materials and supplies in accordance with established policies and procedures, counts orders upon receipt.

• Bachelor's degree in biology, chemistry or related field.

• Some related post-undergraduate work experience.

Classified Title: Research Technologist 
Job Posting Title (Working Title): Research Technologist   
Role/Level/Range: ACRO40/E/02/CC  
Starting Salary Range: $15.70 - $26.25 HRLY ($ 21.00/hour targeted; Commensurate with experience) 
Employee group: Casual / On Call 
Schedule: M-F/8:30-5:00 
Exempt Status: Non-Exempt 
Location: School of Medicine - East Baltimore Campus 
Department name: 10002941-SOM Onc Urologic Oncology 
Personnel area: School of Medicine 

About Johns Hopkins University

Johns Hopkins University remains committed to its founding principle, that education for all students should be grounded in exploration and discovery. Hopkins students are challenged not just to learn but also to advance learning itself. Critical thinking, problem solving, creativity, and entrepreneurship are all encouraged and nourished in this unique educational environment. After more than 130 years, Johns Hopkins remains a world leader in both teaching and research. Faculty members and their research colleagues at the university's Applied Physics Laboratory have each year since 1979 won Johns Hopkins more federal research and development funding than any other university. The university has nine academic divisions and campuses throughout the Baltimore-Washington area. The Krieger School of Arts and Sciences, the Whiting School of Engineering, the School of Education and the Carey Business School are based at the Homewood campus in northern Baltimore. The schools of Medicine, Public Health, and Nursing share a campus in east Baltimore with The Johns Hopkins Hospital. The Peabody Institute, a leading professional school of music, is located on Mount Vernon Place in downtown Bal...timore. The Paul H. Nitze School of Advanced International Studies is located in Washington's Dupont Circle area.

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