The successful applicant will work with a significant degree of independence under the direct supervision of the PI in designing and executing experimental studies in in vitro and in vivo model systems, analyzing the data obtained from these studies, and preparing/formatting these data in whatever context is needed for their dissemination (e.g., publication, grant preparation, funding report submission, etc.). Our research effort is focused on understanding the gene-regulatory and epigenetic mechanisms that govern early cell fate decisions during cardiogenesis and cell state transitions in the setting of adult pathologic cardiac remodeling. We have generated/are generating several novel lines of mice and stem cells that harbor genetic alterations at specific loci which will allow us to investigate the roles for a family of histone acetyl-lysine reader proteins and nuclear tethering factors in these processes. The candidate will require both theoretical and hands-on skills in these areas of research in order to contribute successfully to the growth of the lab. The successful candidate will have the opportunity to eventually take on a lab manager role with additional administrative responsibilities as well as scientific and program management responsibilities.
Specific Job Responsibilities:
* Independently perform culturing and genomic manipulations of mouse embryonic stem (mES) and human induced pluripotent stem (hIPS) cells and directed differentiation to atrial and ventricular cardiomyocytes as well as neural crest cells.
* Independently design, generate, and validate novel mouse genetic alleles that selectively abolish specific functions of a family of histone acetyl reader proteins or a transcription factor important in resident fibroblast to activated myofibroblast cell state transitions.
* Independently perform assessments of gene expression (from bulk tissue and single-cells along with nascent transcription), chromatin occupancy, and chromatin accessibility (from blk tissue and single-cells) including sample collection and processing, library preparation, multiplexed sequencing, and downstream data analysis.
* Independently assess developmental consequences of tissue-specific deletion of a family of histone acetyl reader proteins or a transcription factor important in resident fibroblast to activated myofibroblast cell state transitions.
* Independently perform standard molecular biology and biochemistry techniques (including molecular cloning, quantitative reverse transcription PCR, virus preparation and in vitro/in vivo viral transduction, Western blotting, immunoprecipitation, co-immunoprecipitation, and chromatin immunoprecipitation) as well as high-resolution microscopy (including confocal microscopy and live-cell imaging).
* Perform project management functions for large collaborative projects involving multiple laboratories, oversee the day-to-day tasks and responsibilities of a staff research associate (SRA), and assume responsibility for maintenance of a 150-cage mouse colony.
Required qualifications:
* PhD in Biological Sciences, Bioengineering, or related fields.
* Advanced knowledge of stem cell culture (including mouse embryonic and human induced pluripotent stem cells), standard molecular biology and biochemistry techniques, next-generation sequencing-based approaches to biological interrogations, and in vivo mouse modeling.
* Demonstrated ability to contribute data and technical content to grant proposals.
* Proven skills and experience in independently resolving broad problems that may arise in collection or analysis of laboratory data.
* Self-motivated with ability to work independently and as part of a team. Able to learn effectively and meet deadlines.
* Demonstrated experience and ability to collaborate effectively with all levels of staff; technical, students, faculty and administrators.
* Demonstrated effective communication and interpersonal skills.
* Demonstrated ability to communicate technical information to technical and non-technical personnel at various levels in the organization and to external research and education audiences.
* Proven ability to successfully work on multiple concurrent projects.
* Demonstrate high integrity and professionalism to work with patient data in a HIPAA-compliant and ethically responsible manner.
* Ability to effectively manage own time.
Preferred qualifications:
* Advanced skills, and demonstrated experience associated with one or more of the following: performance and analysis and laboratory-based research including design, modification, and implementation of genome-wide assays to interrogate consequences of alterations in protein structure/function.
* Demonstrated ability to regularly interface with management.
* Interest and proven ability to train and guide the efforts of junior members of the team towards carrying out the broader goals of the laboratory.
* Track record of independent research.
* Completion of post-doctoral fellowship.
Screening of applicants will begin immediately and will continue as needed throughout the recruitment period. Salary will be commensurate with the applicants experience and training.
Please apply online at https://aprecruit.ucsf.edu/JPF04705. Applicants' materials must list current and/or pending qualifications upon submission.
See Table 13B for the salary range for this position. A reasonable estimate for this position is $87,100-$113,900.
