Rutgers, The State University of New Jersey, is seeking a Postdoctoral Fellow for NJMS- Microbiology, Biochemistry & Molecular Genetics / Center for Emerging & Re-emerging Pathogens. This position is under the direction and mentorship of Dr. Samantha Bell, Principal Investigator, the Postdoctoral Fellow will investigate the cell biology underlying interactions between macrophages and Mycobacterium tuberculosis. The major focus is understanding the initiation, progression, and resolution of innate immune responses triggered during infection, including cytosolic DNA sensing and selective autophagy. In addition, proteomics approaches and genetic screens will be employed to identify novel players in these pathways, including host proteins and bacterial factors. Validation of previously identified and newly discovered factors will be performed using a combination of bacterial and host genetics, cell biology, biochemistry, molecular biology, and mouse infection models. The work from these studies will deepen our understanding of the molecular interface between the host macrophage and intracellular M. tuberculosis, enabling the future development of more effective therapies for TB.
Among the key duties of this position are the following:
Characterizes the host response to M. tuberculosis in macrophages and in mouse models of infection.
Designs, optimizes, performs, and analyzes host and bacterial screens using CRISPR libraries and transposon libraries.
Generates M. tuberculosis knockouts, complements, and other necessary bacterial strains.
Generates CRISPR knockouts and express epitope-tagged proteins in macrophages.
Performs cell biology, molecular biology, and biochemistry experiments to monitor M. tuberculosis infection, including live cell imaging, RNA-seq, and protein mass spectrometry.
Conducts experiments safely and effectively in a biosafety level-3 (BSL3) facility, including those involving mouse models of tuberculosis.
Prepares manuscripts for publication, including drafting and editing the text and creating figures from data generated.
Maintains an in depth and up-to-date knowledge of the literature related to field of study.
Minimum Education and Experience:
Doctoral Degree in microbiology, cell biology, biochemistry, molecular biology, immunology, or a related relevant science.
Physical Demands and Work Environment:
This position involves prolonged standing, walking and sitting, as well as use of laboratory equipment.
Must perform complex procedures and demonstrate manual dexterity.
Some stooping, reaching, climbing and bending.
Occasional lifting of 20 pounds to a height of 6½ feet.
Hearing and speech capabilities necessary to communicate with other researchers and staff in person, on the telephone and by e-mail.
Vision capable of viewing gauges, computer monitors, charts, forms, text and numbers for prolonged periods.
Must be available to work flexible hours including weekends and holidays when necessary to meet deadlines.
Rutgers, The State University of New Jersey, is a leading national public research university and the state's preeminent, comprehensive public institution of higher education. Rutgers is dedicated to teaching that meets the highest standards of excellence; to conducting research that breaks new ground; and to turning knowledge into solutions for local, national, and global communities. As it was at our founding in 1766, the heart of our mission is preparing students to become productive members of society and good citizens of the world. Rutgers teaches across the full educational spectrum: preschool to precollege; undergraduate to graduate and postdoctoral; and continuing education for professional and personal advancement. Rutgers is New Jersey's land-grant institution and one of the nation's foremost research universities, and as such, we educate, make discoveries, serve as an engine of economic growth, and generate ideas for improving people's lives.