The Department of Genetic Medicine is seeking a Research Specialist, under the direct supervision of the Laboratory Manager, to assist in experiments and lab maintenance for the laboratory of Dr. Ambroise Wonkam, Director of the Department of Genetic Medicine. The position requires a developed understanding of molecular techniques required to carry out laboratory experiments and the ability to apply basic analytical skills to implement assigned projects.
Specific Duties & Responsibilities
Performs lab tests and experiments as directed by of the Laboratory Manager in support of research related to the study of genetic modifiers of sickle cell disease clinical expressions as well as functional validation of novel variants (in known genes or in novel genes) for congenital hearing impairment.
With minimal supervision, follow established procedures or protocols of assays and wet bench techniques of cell biology and molecular biology including: nucleic acid isolation, quantitative real-time PCR, cell culture, transfection, cloning, gel electrophoresis, next-generation sequencing and gene expression studies.
Independently operates basic laboratory equipment such as an analytical balance, light microscope, cell counter, thermal cyclers, real-time instrumentation and laminar flow hood. This person will also gain experience in flow cytometry and cell sorting.
Will assist in data analysis and begin to learn computational methods to interrogate data emanating from genome-wide association studies. Uses lab computers for data entry and maintains accurate data records.
Following standard laboratory procedures to prepare solutions and aliquot reagents following standard laboratory formulas and procedures. Assistants with preventative maintenance of equipment and maintain documentation of maintenance.
Assist Lab Manager for inventory tracking and ordering.
Use of computer software applications to assemble data.
Responsible for adhering to all policies and procedures set forth by the JHU SOM and the Department of Genetic Medicine, including Administration policies.
Other duties as assigned by Lab Manager and/or PI.
Special Knowledge, Skills & Abilities
Must be familiar with the operation of laboratory equipment such as PCR machines, real-time PCR machines, centrifuge, pH meter, analytical balance, incubator, spectrophotometer, light microscope, imaging systems and laminar flow hoods.
Must be organized and familiar with aseptic technique.
Must be familiar with theory behind experiments and apply analytical skills to interpret experiments, including positive and negative controls.
Specific Physical Requirements
Reaching by extending hand(s) or arm(s) in any direction; Finger dexterity to manipulate objects with fingers rather than with whole hand or arm.
Communication skills using the spoken word.
Ability to see and hear within normal parameters; lift, carry, move supplies, computers, glassware, etc.
Ability to move about workspace.
Bachelor's degree in biology, chemistry or related field.
One year laboratory experience performing relevant techniques.
Master's degree, with related graduate research, may substitute for experience to the extent permitted by the JHU equivalency formula.
Classified Title: Research Specialist Job Posting Title (Working Title): Research Specialist Role/Level/Range: ACRP/03/MA Starting Salary Range: Min $35,200 Max $61,600 ($48,400 targeted; Commensurate with experience) Employee group: Full Time Schedule: M-F 8:30 to 5pm Exempt Status: Non-Exempt Location: School of Medicine Department name: Genetic Medicine Personnel area: School of Medicine
Total Rewards The referenced salary range is based on Johns Hopkins University's good faith belief at the time of posting. Actual compensation may vary based on factors such as geographic location, work experience, market conditions, education/training and skill level. Johns Hopkins offers a total rewards package that supports our employees' health, life, career and retirement. More information can be found here: https://hr.jhu.edu/benefits-worklife/
Please refer to the job description above to see which forms of equivalency are permitted for this position. If permitted, equivalencies will follow these guidelines: JHU Equivalency Formula: 30 undergraduate degree credits (semester hours) or 18 graduate degree credits may substitute for one year of experience. Additional related experience may substitute for required education on the same basis. For jobs where equivalency is permitted, up to two years of non-related college course work may be applied towards the total minimum education/experience required for the respective job.
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