Career Center

We are seeking a highly motivated and experienced Senior Research Technician to conduct basic and translational research in the field of oncology. Research Technician will work in Drs. Nikhil Munshi???s and Kenneth Anderson???s labs in the Jerome Lipper Multiple Myeloma center at Dana-Farber Cancer Institute. Our lab research interest involved multiple myeloma; and our studies have transformed myeloma therapy and patient survival over the last decades through the identification and validation of therapeutic targets in tumor cells and bone marrow microenvironment.

Located in Boston and the surrounding communities, Dana-Farber Cancer Institute is a leader in life changing breakthroughs in cancer research and patient care. We are united in our mission of conquering cancer, HIV/AIDS and related diseases. We strive to create an inclusive, diverse, and equitable environment where we provide compassionate and comprehensive care to patients of all backgrounds, and design programs to promote public health particularly among high-risk and underserved populations. We conduct groundbreaking research that advances treatment, we educate tomorrow's physician/researchers, and we work with amazing partners, including other Harvard Medical School-affiliated hospitals.

The technician will be an integral member of our research team and will assist scientists in performing scientific research, development and/or clinical tasks of varying complexity. Current projects include studies on novel immunotherapeutic approaches for the treatment of myeloma and analysis of effect of experimental drugs in the context of the bone marrow microenvironment. The senior lab technician will be involved in the study of characterization of tumor suppressor genes (TSGs) in myeloma cells. CRISPR activation and ORF expression will be use for gain of function studies, and CRISPR inhibition or shRNA-based genetic depletion will be used for loss of function studies. The biological role of the TSG will be then evaluated using specific assays including cell proliferation and apoptosis assays. Transcriptomic and proteomic changes will be assessed by western blot, q-PCR and sequencing. Transcriptional regulation of the TSG will be also investigated using epigenetic (such as miRNA and lncRNA regulation) and other approaches. Role of TSG will be also investigated in vivo myeloma models.

The role of the senior lab technician serves a very important and critical role in the flow of the laboratory???s research. Previous lab experience is needed as the candidate will be expected to independently carry out essential research functions in the laboratory that include but are not limited to:

• Cell culture
• Western blot analysis (immuno-precipitations and immunoblotting),
• RNA/DNA extractions, PCR and RT-PCR
• Transient transfection and lentiviral transduction for CRISPR-based LOF and GOF studies
• Performing cytotoxicity and proliferation assays
• Flow cytometry assays and experience with analysis (DIVA, FLOW-JO) is preferred.
• In vivo analysis??
• Assists research fellows with various experiments using multiple myeloma cell lines and patient samples
• Attends lab meetings and actively participates in communicating updates on sample intake for various protocols
• Maintains accurate electronic and physical notebooks. ??

SUPERVISORY RESPONSIBILITIES:

No supervisory responsibilities.?? Normally receives general instructions on routine work, detailed instructions on new projects or assignments.

• Bachelor???s Degree required with a major in biology or chemistry preferred.?? Master's Degree preferred
• Typically requires a minimum of 1-4 years related experience.
• ??Knowledge of information and techniques related to either biology or immunology is preferred.
• Must have excellent communication skills (written and verbal) and be able to work with a wide variety of faculty and staff.
• Must have good organizational and interpersonal skills and a working knowledge of data management, word processing, graphic software (Excel, MS Word, GraphPad/PRISM) is preferred.
• Ability to analyze information and use logic to address work-related issues and problems.
• Must possess basic mathematic skillset
• Must have attention to detail and be thorough in completing work tasks.
• Ability to work within a team environment.
• Expected to work full time (40 hours/week) for one year with option to extend position beyond this term.
• The successful candidate is expected to work flexible hours in order to accommodate experimental needs.

At Dana-Farber Cancer Institute, we work every day to create an innovative, caring, and inclusive environment where every patient, family, and staff member feels they belong.?? As relentless as we are in our mission to reduce the burden of cancer for all, we are equally committed to diversifying our faculty and staff.?? Cancer knows no boundaries and when it comes to hiring the most dedicated and diverse professionals, neither do we. If working in this kind of organization inspires you, we encourage you to apply.

Dana-Farber Cancer Institute is an equal opportunity employer and affirms the right of every qualified applicant to receive consideration for employment without regard to race, color, religion, sex, gender identity or expression, national origin, sexual orientation, genetic information, disability, age, ancestry, military service, protected veteran status, or other groups as protected by law.

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