The Department of Neurology has an opening for a Research Technician in the study of hereditary dystonia. The technician will work in a team with some supervision but also work independently. Primary responsibilities will include extraction and quantification of DNA and RNA, genomic/cDNA gel electrophoresis and sequence analysis, interrogation and interpretation of DNA sequence variation in families and populations, quantitative RT-PCR and analysis, maintenance and propagation of mammalian and laboratory cell lines. Background in molecular biology and some computer literacy including data management and analysis is preferred.
PRINCIPAL DUTIES AND RESPONSIBILITIES:
Key responsibilities and major job duties:
Understands and applies basic scientific techniques including maintaining lab notebook, performing basic techniques (i.e. pipetting, cell culture, making solutions)
Works with small team to devise experimental strategies, but is able to carry out experimental work independently.
Performs general mammalian tissue culture, including maintenance and propagation of patient and control cell lines (primary skin fibroblasts, EBV-transformed lymphoblasts, iPSCs) as well as common laboratory cell lines (e.g. HEK293, COS, SH-SY5Y)
Isolates DNA and RNA from cell lines and assesses quantity and quality using Bioanalyzer and Nanodrop.
Designs primers and carries out quantitative RT-PCR on RNA from cells. Analyzes results and carries out statistical analysis on findings.
Designs primers and carries out PCR on DNA. Analyzes results for segregation in families.
Carries out computer analysis of sequence data and alignment with genomic sequence. Interprets functionality of variants using various computer resources including the UCSC genome browser, ExAC browser, Gtex database and other online databases.
Maintain and order supplies
Assists with organizing materials for publication or presentation
Qualifications SKILLS & COMPETENCIES REQUIRED:
Able to think and work independently. Strong organizational skills and an ability to work as part of a team. Analytical skills to interpret data and design follow-up experiments. High degree of computer literacy including data management programs as well as data analysis programs. Demonstrated competence in research techniques and methodologies and has the understanding to modify when encounters problems. Demonstrated knowledge of molecular genetics with bench experience in methods. Strong writing and communication skills.
Bachelor's Degree required. Some background in experimental bench biology.
Research related experience preferred. Some background in standard molecular biological and biochemical methods is also expected.
To order supplies and equipment.
Work will primarily be performed in laboratory space within the Department of Neurology in the MGH research facility in Charlestown. Work will be conducted over a forty-hour work week.
EEO Statement Massachusetts General Hospital is an Affirmative Action Employer. By embracing diverse skills, perspectives and ideas, we choose to lead. All qualified applicants will receive consideration for employment without regard to race, color, religious creed, national origin, sex, age, gender identity, disability, sexual orientation, military service, genetic information, and/or other status protected under law. We will ensure that all individuals with a disability are provided a reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment.Partner's Healthcare is acting as an Employment Agency in relation to this vacancy.
MGH Institute of Health Professions, founded by Massachusetts General Hospital in 1977, is an innovative and independent graduate school in Boston that is a member of Partners HealthCare. A progressive leader in developing comprehensive models of health care education, the MGH Institute prepares advanced practice professionals in the fields of nursing, physical therapy, occupational therapy and communication sciences and disorders through a distinctive combination of academic study, clinical practice, and research. More than 1,200 students are enrolled in graduate level and certificate programs, with an increasing number of courses available online. The Institute is accredited by the New England Association of Schools and Colleges (NEASC).