The Knipe laboratory studies mechanisms of lung diseases such as acute lung injury and pulmonary fibrosis. Our lab is especially interested in the role of the endothelium in the development of pulmonary fibrosis and conducts several projects involving both translational human studies and mouse models of disease. The individual hired for this position will have some experience with basic cell biology techniques (ideally gene expression by PCR and protein expression by Western blotting) and a high degree of computer literacy. This person will be involved in translational human and mouse studies using a variety of molecular and cellular techniques including, but not limited to: flow cytometry, single-cell sorting, DNA and RNA extraction, RNA sequencing, PCR and real-time PCR analysis, cell isolation and culture, and immunohistochemistry. This person will also assist with mouse studies, including disease modeling, surgery/procedures, husbandry, and cell culture. There will also be opportunities to help collect and process human lung tissue from lung transplantations to translate into various culture studies in the lab, which will require interfacing with MGH pathology. This position is an excellent opportunity to work with a collaborative and collegial group of lung biology researchers focuses on developing novel anti-fibrotic therapies.
PRINCIPAL DUTIES AND RESPONSIBILITIES:
Please note, the functions below are representative of major duties that are typically associated with these positions. Specific responsibilities may vary based upon departmental needs. Similarly, not all duties that have been outlined will be assigned to each position.
Prepares basic solutions and performs base-level procedures as assigned (i.e. - pipetting, cell and tissue culture, etc.)
Maintains laboratory notebook
Understands and applies basic scientific techniques
Conducts analysis of results and may begin interpretation of results
Sets up and prepares routine experiments as directed
Prepares lab reagents, chemicals, instruments and equipment
May perform independent literature searches
Assists with organizing materials for publication or presentation
Maintains and orders supplies
A Research Technician II performs the duties of a Research Technician I (above) and may also:
Prepares and runs samples on flow cytometer
Writes and executes protocols of non-routine experiments.
Interface with Pathology to collect and process Tissue from Lung Transplantation.
Participates in the design of experiments and assist post-doctoral fellows with determining the most suitable methodology.
Organizes, analyzes and summarizes acquired data, using scientific and statistical techniques.
Organizes and/or drafts data for the preparation of research papers, manuscripts, or other documents for publication and/or presentation.
Attend research meetings and present data.
Demonstrated competence in basic research techniques and methodologies: Familiarity with basic molecular biology techniques, ability to work with mice.
Ability to work independently and as a team member
Analytical skills and ability to resolve technical problems.
Ability to interpret acceptability of data results.
Working knowledge of data management programs. (Microsoft Excel required; GraphPad Prism, FlowJo, and R/Python desirable).
High degree of computer literacy.
Bachelor's degree required.
Some experience performing research in a laboratory setting, either while a student, during summers, or following graduation. Experience in cell and molecular biology preferred.
SUPERVISORY RESPONSIBILITY (if applicable):
A Research Technician II may serve as a team leader to lab assistants and Research Technician I's.
EEO Statement Massachusetts General Hospital is an Affirmative Action Employer. By embracing diverse skills, perspectives and ideas, we choose to lead. All qualified applicants will receive consideration for employment without regard to race, color, religious creed, national origin, sex, age, gender identity, disability, sexual orientation, military service, genetic information, and/or other status protected under law. We will ensure that all individuals with a disability are provided a reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment.Partner's Healthcare is acting as an Employment Agency in relation to this vacancy.
MGH Institute of Health Professions, founded by Massachusetts General Hospital in 1977, is an innovative and independent graduate school in Boston that is a member of Partners HealthCare. A progressive leader in developing comprehensive models of health care education, the MGH Institute prepares advanced practice professionals in the fields of nursing, physical therapy, occupational therapy and communication sciences and disorders through a distinctive combination of academic study, clinical practice, and research. More than 1,200 students are enrolled in graduate level and certificate programs, with an increasing number of courses available online. The Institute is accredited by the New England Association of Schools and Colleges (NEASC).