Conducts tests and procedures; sets up and operates equipment; records observations and measurements and collects and compiles data in a research lab; requires a practical understanding of a limited number of standard scientific techniques.
Please note: Willingness to work with mice, willingness to occasionally work evenings or weekends if experiments require
Specific Responsibilities:
Follows pre-defined protocols, policies, procedure, regulations, Good Laboratory Practices and conducts experiments and completes basic trouble- shooting techniques.
Sets-up, operates and maintains standard lab equipment.
Using an entry level of skill, completes the following activities (please highlight):
Molecular Biology:
DNA, RNA, Protein isolation, purification and expression
Southern, Northern, Western blotting
PCR, RT-PCR
Plasmid preparation
Cloning, mapping and sequencing of DNA
Recombinant virus production and purification
Histology:
Tissue fixation/processing: embedding, preparation of blocks and sectioning, staining
Immunohistochemistry
Microscopy
Immunology:
Flow cytometry
ELISA, Elispot assay
Cytokine assay
Antibody production and purification
Animal:
Husbandry
Breeding and screening transgenic mice
Dissections
Blood collection
Surgical procedures
Database management of colony
Records test results and may maintain databases.
Completes standard and routine clerical duties such as copying, filing, data entry, word processing, etc.
Maintains inventory of reagents, mice and lab supplies
Miscellaneous
Performs other duties as assigned.
Minimum Qualifications:
HS diploma or equivalent and some basic science courses is required.
Minimum Competencies: (Skills, knowledge, and abilities.)
Demonstrated laboratory experience in molecular biology;
Demonstrated ability to follow detailed instructions as required by research protocols;
Demonstrated computer skills, including spreadsheets and basic computations;
Attention to detail and accurate record keeping;
Ability to work independently and in a team environment;
Manual dexterity.
Ability to work with animals (mice).
Preferred Qualifications:
Mouse handling and colony management, molecular biology, gene expression analysis, good organizational skills
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