St. Petersburg, Florida
Northern & Central California, California
Cambridge, Massachusetts
Work Remote,
New York, New York
New Orleans, Louisiana
New York, New York
Posted: 17-May-23
Location: Baltimore, Maryland
Salary: Open
Internal Number: 109234-en_US
The Johns Hopkins University is seeking a highly motivated Research Specialist II who is interested to join our team to work on the in vitro and in vivo testing of brain gene therapy candidates. The Research Specialist II will participate in the development and testing of gene transfer methods for the mouse brain. She/he will be working in a team that includes other technicians, graduate students and postdoctoral fellows and will make significant and important contributions to various aspects of these research eff orts. This will include neuronal cultures, rodent injections, genotyping, gene expression measurement qPCR, and immunoblotting/immunohistochemistry for protein expression. Our laboratory investigates the mechanisms of synaptic plasticity and how defects in this process cause intellectual disability, autism, and epilepsy. We use a wide spectrum of techniques (molecular biology, biochemistry, cell biology, mouse genetics, histology, in vitro/in vivo imaging, omics studies) to study sensory the neurons that support learning and intelligence.
The individual will contribute through cell culture, CRISPR/Cas9 genetic engineering, FACS, sequencing, genotyping, qPCR, in vitro/vivo electroporation, and immunoblotting/immunohistochemistry towards the overall goal of advancing genetic modification tools and generating genetically modified marmosets.
Will work in an interdisciplinary Marmoset Working Group at Hopkins funded by the BRAIN Initiative (U01 DA056556) with broad expertise in embryology, reproductive techniques, genetic engineering, and neuroscience.
Specific Duties & Responsibilities
- Make scientific contribution to protocol development, efficiency, and productivity.
- Apply published research methods to current experimental work by investigating published protocols and discussing them with PI.
- Maintain detailed working knowledge of all assigned protocols.
- Collaborate with lab members in development of new techniques and instruments.
- Assistance with the maintenance of mouse colonies.
- Participate in the development and implementation of lab goals and objectives.
- Analyze data and process analyzed data into figures for scientific publications.
- Assist with the development and administration of laboratory budget.
- Contribute toward the efficient utilization of resources, equipment and space.
- Manage inventory, monitor supply levels, order equipment and supplies in accordance with established policies and procedures, and initiate the process for equipment repair when needed.
- Teach/train students, and new technicians in the lab. Guide, direct, and facilitate problem solving for entry-level lab members on day-to-day problems.
- Perform miscellaneous related duties as assigned.
- Work on CRISPR/Cas9 genetic editing of stem cells and the generation of mutant non-human primate models for neuroscience.
- Participate in developing and testing genetic editing methods applicable to stem cells and embryos.
Minimum Qualifications
- Bachelor's Degree in Biology, Chemistry or related field.
- Two years laboratory experience performing relevant techniques.
- Master's degree, with related graduate research, may substitute for experience to the extent permitted by the JHU equivalency formula.
Preferred Qualifications
- Prior experience with stem cells, CRISPR/Cas9 genetic engineering, molecular biology techniques, DNA/RNA sequencing techniques, electroporation, and imaging.
- Able to work well independently and as part of a team.
- Strong organizational skills.
- Problem-solving skills.
Classified Title: Research Specialist II
Role/Level/Range: ACRP/03/MB
Starting Salary Range: $40,100-$55,140-$70,180 Annually (Commensurate with experience)
Employee group: Full Time
Schedule: 5/37.5
Exempt Status: Exempt
Location: School of Medicine Campus
Department name: SOM Neuro Sci Neuroscience
Personnel area: School of Medicine
Total Rewards
The referenced salary range is based on Johns Hopkins University's good faith belief at the time of posting. Actual compensation may vary based on factors such as geographic location, work experience, market conditions, education/training and skill level. Johns Hopkins offers a total rewards package that supports our employees' health, life, career and retirement. More information can be found here: https://hr.jhu.edu/benefits-worklife/
Please refer to the job description above to see which forms of equivalency are permitted for this position. If permitted, equivalencies will follow these guidelines:
JHU Equivalency Formula: 30 undergraduate degree credits (semester hours) or 18 graduate degree credits may substitute for one year of experience. Additional related experience may substitute for required education on the same basis. For jobs where equivalency is permitted, up to two years of non-related college course work may be applied towards the total minimum education/experience required for the respective job.
**Applicants who do not meet the posted requirements but are completing their final academic semester/quarter will be considered eligible for employment and may be asked to provide additional information confirming their academic completion date.
The successful candidate(s) for this position will be subject to a pre-employment background check. Johns Hopkins is committed to hiring individuals with a justice-involved background, consistent with applicable policies and current practice. A prior criminal history does not automatically preclude candidates from employment at Johns Hopkins University. In accordance with applicable law, the university will review, on an individual basis, the date of a candidate's conviction, the nature of the conviction and how the conviction relates to an essential job-related qualification or function.
The Johns Hopkins University values diversity, equity and inclusion and advances these through our key strategic framework, the JHU Roadmap on Diversity and Inclusion.
Equal Opportunity Employer
All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran.
EEO is the Law
Learn more:
https://www.eeoc.gov/sites/default/files/migrated_files/employers/poster_screen_reader_optimized.pdf
Accommodation Information
If you are interested in applying for employment with The Johns Hopkins University and require special assistance or accommodation during any part of the pre-employment process, please contact the Talent Acquisition Office at jhurecruitment@jhu.edu. For TTY users, call via Maryland Relay or dial 711. For more information about workplace accommodations or accessibility at Johns Hopkins University, please visit accessibility.jhu.edu.
Johns Hopkins has mandated COVID-19 and influenza vaccines, as applicable. Exceptions to the COVID and flu vaccine requirements may be provided to individuals for religious beliefs or medical reasons. Requests for an exception must be submitted to the JHU vaccination registry. For additional information, applicants for SOM positions should visit https://www.hopkinsmedicine.org/coronavirus/covid-19-vaccine/ and all other JHU applicants should visit https://covidinfo.jhu.edu/health-safety/covid-vaccination-information/.
The following additional provisions may apply, depending on campus. Your recruiter will advise accordingly.
The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.
Note: Job Postings are updated daily and remain online until filled.
