St. Petersburg, Florida
Northern & Central California, California
Cambridge, Massachusetts
Work Remote,
New York, New York
New Orleans, Louisiana
New York, New York
Posted: 13-May-23
Location: La Jolla, California
Salary: Open
Internal Number: 123155
UCSD Layoff from Career Appointment: Apply by 5/15/23 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 5/26/23. Eligible Special Selection clients should contact their Disability Counselor for assistance.
Job posting will remain open until a suitable candidate has been identified.
DESCRIPTION
The Staff Research Associate will carry out research under the supervision of the Principal Investigator (PI) on the intersection of variation in viral populations and the induction of an innate immune response. Incumbent will assist the Principal Investigator by performing molecular, biological, biochemical, and cell biological techniques, including cell culture, mammalian cell transfection, culturing of mammalian viruses, mammalian cell transduction, DNA cloning, DNA and RNA isolation and analysis, PCR, qRT-PCR, RNAseq, and flow cytometry. Occasionally perform other standard repetitive techniques as required.
Incumbent will be required to participate in lab meetings and seminars related to the projects.
The position responsibilities also encompass a variety of laboratory maintenance duties that include organizing and keeping records of reagents in freezers, refrigerators, and cryostorage, managing an inventory of all lab supplies, and standard upkeep of lab equipment. Perform other duties as assigned.
QUALIFICATIONS
Theoretical and practical background and knowledge in Molecular Biology, Cell Biology, Microbiology, Genetics, or Biochemistry as is typically acquired by a Bachelor's degree and/or by a few years of lab experience in any of the related fields of study.
Demonstrated ability to follow and troubleshoot established lab protocols and lab procedures with direction from PI.
Excellent organizational skills including the ability to keep clear and accurate records, organize a varied workload, follow written and oral protocols and give oral presentations on the progress of research in lab meetings. Proven ability to prioritize and schedule experiments and duties to maximize efficiency.
Excellent interpersonal communication skills to interact well with others to facilitate communication about lab requirements including the ability to discuss and understand day-to-day laboratory affairs, research plans, results and ideas with PI and other lab personnel in a constructive, professional and pleasant manner.
Experience in sterile technique as applied to the propagation of prokaryotic and mammalian cells.
Experience and knowledge of proper handling, storage and disposal of chemicals, biochemicals, hazardous chemicals, and biohazardous materials.
Experience in cloning and recombinant expression procedures. Proficiency in gene expression analysis techniques, such as quantitative RT-PCR.
Familiarity with flow cytometry (including cell staining and data analysis).
Skill and demonstrated ability to operate standard laboratory equipment, including centrifuges, balances, gel electrophoresis equipment, and pH meters.
Demonstrated skill and knowledge to proactively maintain an organized lab and stocks of essential supplies and media, purchase new equipment, and ensure lab equipment is operating effectively.
Knowledge/understanding and at least some experience with Safety Data Sheets, hazard communication and Injury and Illness Prevention Program. Familiarity with a Chemical Hygiene Plan addressing safety precautions including appropriate clothing and shoes, safe handling of chemicals, use of protective items. Willingness and ability to keep safety training current and consistent with research involvement.
Basic mathematical skills; knowledge of accounting and billing practices, etc. Knowledge of purchasing and reimbursement procedures. Ability to proactively and appropriate facilitate the maintenance of all lab and equipment, maintain steady levels of lab supplies and purchasing records.
Experience using Apple, PC computers and associated word processing and spreadsheet software with a willingness and ability to update and increase skills and knowledge as needed.
Willingness and demonstrated ability to embrace and consistently uphold the principles of community for a collaborative work environment.
SPECIAL CONDITIONS
Must be willing and able to work safely with BL2 level bacterial and viral preparations, including influenza virus.
Flexible work schedule required. Willingness and ability to work occasional evenings and/weekends as required by the needs to the research project(s).
