Northern & Central California, California
Cambridge, Massachusetts
Work Remote,
New York, New York
California
New Orleans, Louisiana
New York, New York
Los Angeles, California
Posted: 02-May-23
Location: La Jolla, California
Salary: Open
Internal Number: 118665
This position will remain open until a successful candidate has been identified.
UCSD Layoff from Career Appointment: Apply by 09/08/2022 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 09/16/2022. Eligible Special Selection clients should contact their Disability Counselor for assistance.
Placement within the range is determined by internal equity, relevant qualifications, and collective bargaining agreements (when applicable).
DESCRIPTION
The Moores Cancer Center is one of just 51 in the United States to hold a National Cancer Institute (NCI) designation as a Comprehensive Cancer Center. As such, it ranks among the top centers in the nation conducting basic and clinical cancer research, providing advanced patient care and serving the community through outreach and education programs. The Cancer Center's mission is to translate promising scientific discoveries into new and better options for the care of patients with cancer. The Center supports one of the broadest ranges of cancer activities in the nation – from fundamental and translational cancer research in molecular genetics and advanced molecular therapeutics, and from population studies of cancer incidence to community education about cancer prevention and risk reduction.
Under supervision, assist with performing research techniques and research and development studies to optimize the isolation, expansion and modification of patient-derived T cells and to develop methodologies under current good manufacturing conditions (cGMP) to re-administer the cells to the donor patients.
Assist Cancer Immunotherapy Program (CIP) personnel with establishing scalable procedures to expand and transfect T-cells to form CAR-T cells and support the preclinical and clinical development of these drug candidates. These engineered T-cells will be created by the employment of a number of modern molecular biology techniques including cell transduction and transfection techniques that employ viral and nucleic acid vectors. Assist personnel with generation of clinical grade viral vector systems and the production of gene delivery systems that will be transitioned into a cGMP facility. Other duties assigned as needed.
MINIMUM QUALIFICATIONS
Theoretical knowledge of cell biology, immunology, biotechnology, and/or stem cell biology.
Theoretical and practical knowledge of biochemistry, molecular biology, immunology and genetics including relevant techniques. Knowledge of flow cytometry and FACS.
Experience with sterile technique and the ability to perform cell culture.
Experience with cellular immunology and/or tumor biology.
Ability to work with cellular transfection/transduction methods.
Ability to work with in vivo or in vitro assays systems, e.g. ELISA, Cytokine determination, ELISPOT, proliferation and cytotoxicity assays.
Demonstrated experience working in and maintaining a laboratory environment.
Proven ability to accurately keep records regarding cell lines, source, passage, and genetic manipulations. Proven ability to independently organize, analyze, and interpret data.
Strong communication skills (both verbal and written) needed to interact professionally and effectively in the work environment, as well as effectively and accurately document research procedures. Ability to read, comprehend and discuss research materials.
Experience with the operation of a personal computer, servers and software applications, such as MS Word and Excel. Knowledge to work with and learn new software for analysis.
Ability to follow UCSD Environmental Health and Safety requirements including the ability to write with correct syntax and grammar and to clearly communicate ideas and protocols in writing to EH&S.
PREFERRED QUALIFICATIONS
- Ability to work with primary mammalian cell culture and with PBMC (Peripheral Blood Mononuclear Cells).
- Enthusiasm for learning new techniques and a genuine interest in scientific research. Willingness and ability to learn new skills, research methods, and scientific principles. Ability to train students on same.
- Experience with T-Cell biology. Experience isolating primary human T-cells from blood products using both manual and automated systems for sterile cell culture. Experience transducing and immunophenotyping primary human T-cells using aseptic technique.
SPECIAL CONDITIONS
- Employment is subject to a criminal background check.
- Must be able to maintain confidenti Connections working at University of California San DiegoUC San Diego is an academic powerhouse and economic engine, recognized as one of the top 8 public universities by U.S. News and World Report. Innovation is central to who we are and what we do. Here, students learn that knowledge isn't just acquired in the classroom - life is their laboratory. UC San Diego's rich academic portfolio includes six undergraduate colleges, five academic divisions and five graduate and professional schools. The university's award-winning scholars are experts at the forefront of their fields with an impressive track record for achieving scientific, medical and technological breakthroughs.
