The Conklin Lab is looking for a Research Associate I or II. The applicant will support laboratory research projects on human therapeutic gene editing in close collaboration with the Innovative Genomics Institute. The goals of the projects are to explore CRISPR based gene editing strategies in neurons as well as the development of in vitro models of neurodegenerative disease using iPSC derived neuronal cells. Strong communication skills, with a background in molecular biology and cell culture preferred.Â Applicants intending on progressing to medical or graduate school are encouraged to apply. Â Â
Please submit a resume/CV and Statement of Interest. Â
About the Lab:
In The Conklin lab we focus on allele-specific gene editing as a therapeutic strategy for human disease.Â We focus ongene mutations that are highly penetrant, with clear phenotypes in cell types that can be readily derived from iPSCs. The diseased cell types allow us to decode the cellular signatures of disease and determine if editing the disease allele restores cellular functioning. Genome editing is a rapidly advancing field that uses state-of-the-art techniques that pushes the boundaries of cell and molecular biology. We use advanced microscopy, tissue engineering and single cell genomics to optimize precise editing. We are developing computational methods to select optimal CRISPR/Cas9 combinations in diverse populations. We aim to produce therapies that are safe and cost effective so that they can benefit the maximal number of people. In collaboration with clinical scientists and the Innovative Genomics Institute we are preparing large animal models and clinical grade reagents to prepare for human clinical trials.
Duties and Responsibilities:
The candidate will be directly supervised by a Senior Scientist or Postdoctoral Fellow and have regular meetings with the PI, Dr. Bruce Conklin.
The Main responsibilities will include but are not limited to:
Human pluripotent stem cell culture, including transfection, subcloning, and differentiation into neurons
Molecular biology assays including nucleic acid extraction, genotyping of human samples by PCR and ddPCR, and sequencing
Molecular cloning for generating new plasmid constructs, ligation, transformation, and plasmid purification
Production of lentivirus and viral like particles
Maintaining stocks, reagents, and laboratory equipment
Communication of original findings in the form of lab meeting presentations
BA/BS in molecular biology, cellular biology or a related science and one or more years of recent, directly related work experience, utilizing the techniques or methods required by the position; and/or equivalent experience/training.
Knowledge and experience with molecular biology techniques (PCR, molecular cloning, sequencing)
Knowledge of scientific equipment (centrifuge, pipettes, thermocyclers, etc.) and basic lab etiquette.
Strong communication skills with history of presenting a scientific group meetings
Attention to detail and meticulous record keeping abilities.
Flexibility in schedule- ability to work evenings and weekends when necessary
Familiarity or interest in human genetics/genetic disease and/or CRISPR/Cas gene editing.
Human or mammalian cell culture experience
Willingness to work with animals or animal tissue
Experience with CRISPR/Cas gene editing
Experience with microscopy, especially epifluorescence microscopy
The Gladstone Institutes is an independent, not-for-profit research institution affiliated with the University of California San Francisco (UCSF), transforming the health and well-being of all people through medical research, education, and outreach in the fields focused on heart disease, immunology and virology including HIV/AIDS, and neurological disease. Home to notable leaders in the field of medical science, including 2012 Nobel Prize in Physiology or Medicine recipient Shinya Yamanaka, M.D.,Ph.D. Gladstone is comprised of four institutes in addition to various Centers and programs focusing approximately 500 members of our team on its mission: Science Overcoming Disease. Located in an award-winning building adjacent to UCSF's Mission Bay Campus, Gladstone offers a competitive salary and benefits program within a robust environment for those seeking to maximize their potential. Please consider Gladstone and make a difference in human health.