Location: Iowa City, Iowa
The Research intern will be responsible for executing scientific research at the Anatomy and Cell Biology department in the gene therapy laboratory directed by John Engelhardt, under the supervision of postdoctoral scientists and graduate students. The candidate will be a highly motivated individual who is curious about basic scientific questions, enjoys learning in a challenging academic environment and integrates effectively into the team. The candidate is expected to occasionally report to industry partners working with Dr. Engelhardt. US citizenship or permanent residency is required. More information on the Research Internship program can be found here: http://research.uiowa.edu/post-baccalaureate-research-internship
Research: Performs day-to-day experimental activities, maintains detailed up-to-date laboratory notebook, utilizes safe laboratory and animal protocols. The candidate will perform activities based upon protocols developed by other research staff of the principal investigator of the research project. Read and review articles as directed. As individual skills develop, analyze literature to develop hypotheses for discussion with other researchers or principal investigator.
Experimental Research and Evaluations: Read and understand protocols. Arrange and plan daily activities to around setup of materials or equipment. Perform procedures assuring fidelity to protocols. Perform varied tasks in the research environment. Collect, record and consolidate data as directed by the Principal Investigator. Track progress of research activities. Adhere to Quality Assurance protocols to maintain validity and integrity of research data. As individual skills develop: troubleshoot, analyze and propose modifications to protocols; perform complex techniques per protocol; test and evaluate current procedures; develop and implement procedures for monitoring data integrity; may present results at team meetings.
In vitro: Mammalian and human cell culture, real-time qPCR, RNA tissue extraction, DNA sequencing, CRISPR/Cas9 technology, electrophysiology, immunohistochemistry, neutralization test, ELISpot and ELISA.
In vivo: Animal handling of small and large mammals, knowledge of animal anatomy, tissue collection from virus-infected tissue and tissue analysis.