Associate Project Scientist University of California Los Angeles
Requisition Number: JPF08090
Applications are sought for a full-time Assistant Project Scientist position. The successful candidate will work as part of an interdisciplinary research team in the laboratory of Dr. Kathrin Plath's Lab in the UCLA Department of Biological Chemistry. The focus of the Plath Lab is on understanding the gene regulatory mechanisms underlying cellular plasticity, reprgramming and differentiation to open the door to manipulate cell identity and gene expression states and to understand disease processes. Within this context, the Plath lab is studying six fundamental problems: (i) How do long-noncoding (lnc) RNAs build nuclear compartments and regulate chromatin changes during differentiation and reprogramming? This aspect of our work particularly centers on understanding how the X chromosome dosage compensation is regulated by the lncRNA Xist in mouse and human. (ii) Which mechanisms govern reprogramming to induced pluripotency as a means to understand how transcription factors drive cell fate changes and induce new chromatin and gene expression states. (iii) How transcriptional networks and lncRNAs regulate 3D genome organization, and how 3D genome organization in turn influences gene expression, chromatin states, and lncRNA function. (iv) How nutrients that vary with diet can regulate stem cell states and cell fate decisions, particularly those during development, with the ultimate goal to define dietary alterations that enable more accurate models of differentiation in the culture dish or lead to new treatments of pregnancy complications and diseases. (v) Using embryonic stem cells, induced pluripotent stem cells and new embryo models to understand the gene regulatory networks underlying cellular specification during development, implantation and in early pregnancy and to understand how things can go wrong. (vi) Developing new single cell genomics technologies and to apply them to diverse biological model systems to, for instance, understand the difference between the healthy and disease lung.
To address these questions, the Plath lab is using a combination of cell culture, microscopy, biochemistry and mass spectrometry, high-throughput screening, transgenic/knockout mouse models and pluripotent stem cell models, new embryo models, genome engineering, metabolomics, and bulk and single cell genomics approaches, and participate in fruitful collaborations across campuses. The ideal candidate will have an interest in pursuing a project that addresses how X chromosome dosage compensation is regulated during reprogramming, how autophagy contributes to the reprogramming process, and how genetic variation can be exploited to uncover mechanisms underlying cell fate change. The ideal candidate will be motivated to learn and implement a variety of cutting-edge experimental techniques, and will be excited to work with and develop teams within the lab. Projects may include but are not limited to interrogating mechanisms of reprogramming of somatic cells to iPSCs, characterizing the regulation of Xist RNA in the X-inactivation process, and understanding the role of mitophagy regulators in reprogramming to iPSCs.
The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age or protected veteran status. For the complete University of California nondiscrimination and affirmative action policy, see: UC Nondiscrimination & Affirmative Action Policy, https://policy.ucop.edu/doc/4000376/DiscHarassAffirmAction jeid-9d909d84bddcc5469c76612638e5dd0b
UCLA is known worldwide for the breadth and quality of its academic, research, health care, cultural, continuing education and athletic programs. UCLA offers undergraduate degrees in more than 127 majors and graduate degrees in 198 program areas. UCLA has 11 highly regarded professional schools. Eight are ranked among the nation's top 15 in their field by U.S. News & World Report. UCLA is consistently among the most popular campus in the nation for undergraduate applicants. The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to age, race, color, religion, sex, national origin, disability, or protected veteran status. Benefits:UCLA offers a comprehensive benefits package, including an average of three weeks' vacation per year; an average of 12 days per year sick leave; 13 paid holidays per year; health, dental and optical benefits; life insurance; disability insurance; the University of California Retirement Plan with 5 year vesting and various voluntary UC Savings Plans. There are also special programs and privileges available, such as accessibility to cultural and recreational programs, athletic events, and the University Credit Union.