Postdoctoral Fellow (New Systems Biology & Microbiome Project)
Lawrence Berkeley National Laboratory
Internal Number: 97393
Berkeley Lab's Environmental Genomics and Systems Biology (EGSB) Division has an opening for a Postdoctoral Fellow to join a new Systems biology and microbiome project.The research will focus on the use of methanotrophic bacteria in reducing methane emissions in rice fields and wetlands.
In this exciting role, you will have the opportunity to develop new genetic tools for non-model bacteria, including those from the plant rhizosphere and field soil and apply these tools to characterize microbial gene function on a massive scale. A recent example of tools developed by the team includes random barcode transposon site sequencing (RB-TnSeq; PMID 2596844), dual barcoded shotgun expression library screening (Dub-seq; PMID 30659179), and magic pools for parallel testing of genetic systems in bacteria (PMID 29359196).
Additionally, you will have the opportunity to work on novel microbial isolates from rice fields and be responsible for the establishment of methodologies that will enable the genome engineering using RB-TnSeq to generate gene mutant libraries, sequencing, and analysis of mutant libraries in plant-microbe experiments in a high throughput manner. Performing plant-microbe bioassays and analyzing sequencing data, you will be expected to present your findings in conferences and in peer-reviewed journals.
Lastly, you will have the opportunity to work with a diverse team based at Berkeley Lab in addition to external collaborators, including those in the ENIGMA and m-CAFEs Scientific Focus Areas (SFAs).
What You Will Do:
Culture and genetically engineer diverse bacteria.
Generate and analyze large-scale genetic datasets.
Design and construction of DNA plasmids.
Create, design, and execute plant-microbe experiments using barcoded libraries of transposon mutants in diverse microorganisms to investigate metabolism and gene regulation.
Perform fluorescence microscopy.
Isolate novel microbes from field soil. Apply bioinformatics tools for the analysis of large-scale genomic/metagenomic data.
Infer the functions of previously uncharacterized genes and pathways in diverse bacteria using high-throughput genetics data.
Publish in peer review journals and deliver scientific presentations at scientific meetings.
Effectively communicate and collaborate with a team of experimental and computational scientists on experimental design, data analysis, and long-term project planning.
Keep a detailed laboratory notebook and upload data in relevant databases.
Participation and reporting in research group meetings.
What is Required:
A recent PhD in Molecular Biology, Microbiology, Biochemistry, Genetics, or a related discipline.
Experience with microbiology techniques including culturing bacteria.
Knowledge of molecular biology techniques such as PCR, qPCR, cloning, whole genome amplification.
Knowledge in microbial ecology, physiology, biochemistry, biogeochemistry, molecular biology and understanding of key and complex biological concepts (genes, pathways, microbial phylogeny).
Experience with UNIX utilities and filesystems, R statistical analysis platform, and standard bioinformatics tools and databases.
Excellent oral and written communication skills including the ability to organize/present technical reports and provide a proven track record of recent or pending scientific publications.
Demonstrated interpersonal skills including experience collaborating with a diverse interdisciplinary research team.
What We Prefer:
Experience in any of the following areas: microbial genomics, metagenomics, including data processing/ analysis.
Experience with next generation sequencing platforms (Illumina, PacBio, ONT).
Familiarity with command line sequence analysis tools (e.g., Last, Hmmer, Megahit, MetaSpades, etc.).
For full consideration, please apply by February 15, 2023 with a Curriculum Vitae (CV) or Resume.
This is a full-time 2 year, postdoctoral appointment with the possibility of renewal based upon satisfactory job performance, continuing availability of funds and ongoing operational needs. You must have less than 3 years of paid postdoctoral experience.
The monthly salary range for this position is $4636 - $5995 and is expected to start at $5011 or above. Postdoctoral positions are paid on a step schedule per union contract and salaries will be predetermined based on postdoctoral step rates. Each step represents one full year of completed post-Ph.D. postdoctoral and/or related research experience.
This position may be subject to a background check. Any convictions will be evaluated to determine if they directly relate to the responsibilities and requirements of the position. Having a conviction history will not automatically disqualify an applicant from being considered for employment.
Work will be primarily performed at: West Berkeley Biocenter (Potter St.) -- Bldg. 977, 717 Potter St., Berkeley, CA.
Learn About Us:
Berkeley Lab (LBNL) addresses the world's most urgent scientific challenges by advancing sustainable energy, protecting human health, creating new materials, and revealing the origin and fate of the universe. Founded in 1931, Berkeley Lab's scientific expertise has been recognized with 16 Nobel prizes. The University of California manages Berkeley Lab for the U.S. Department of Energy's Office of Science.
Berkeley Lab's Postdoc Program is committed to providing Postdoctoral Researchers and Visiting scholars with a positive and impactful experience to jump-start their career through premium research and career development, networking opportunities, mentoring programs, and a strong community. For more information, please visit our Berkeley Lab Postdoc Resources site and our Berkeley Lab Postdoc Association site.
Based on University of California Policy - SARS-CoV-2 (COVID-19) Vaccination Program and U.S Federal Government requirements, Berkeley Lab requires that all members of our community obtain the COVID-19 vaccine as soon as they are eligible. As a condition of employment at Berkeley Lab, all Covered Individuals must Participate in the COVID-19 Vaccination Program by providing proof that vaccination requirements have been met or submitting a request for Exception or Deferral. Visit covid.lbl.gov for more information.
Berkeley Lab is committed to Inclusion, Diversity, Equity and Accountability (IDEA) and strives to continue building community with these shared values and commitments. Berkeley Lab is an Equal Opportunity and Affirmative Action Employer. We heartily welcome applications from women, minorities, veterans, and all who would contribute to the Lab's mission of leading scientific discovery, inclusion, and professionalism. In support of our diverse global community, all qualified applicants will be considered for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, or protected veteran status.
In the world of science, Lawrence Berkeley National Laboratory (Berkeley Lab) is synonymous with excellence. Thirteen scientists associated with Berkeley Lab have won the Nobel Prize. Fifty-seven Lab scientists are members of the National Academy of Sciences (NAS), one of the highest honors for a scientist in the United States. Thirteen of our scientists have won the National Medal of Science, our nation's highest award for lifetime achievement in fields of scientific research. Eighteen of our engineers have been elected to the National Academy of Engineering, and three of our scientists have been elected into the Institute of Medicine. In addition, Berkeley Lab has trained thousands of university science and engineering students who are advancing technological innovations across the nation and around the world. Berkeley Lab is a member of the national laboratory system supported by the U.S. Department of Energy through its Office of Science. It is managed by the University of California (UC) and is charged with conducting unclassified research across a wide range of scientific disciplines. Located on a 200-acre site in the hills above the UC Berkeley campus that offers spectacular... views of the San Francisco Bay, Berkeley Lab employs approximately 4,200 scientists, engineers, support staff and students. Its budget for 2011 is $735 million, with an additional $101 million in funding from the American Recovery and Reinvestment Act, for a total of $836 million. A recent study estimates the Laboratory's overall economic impact through direct, indirect and induced spending on the nine counties that make up the San Francisco Bay Area to be nearly $700 million annually. The Lab was also responsible for creating 5,600 jobs locally and 12,000 nationally. The overall economic impact on the national economy is estimated at $1.6 billion a year. Technologies developed at Berkeley Lab have generated billions of dollars in revenues, and thousands of jobs. Savings as a result of Berkeley Lab developments in lighting and windows, and other energy-efficient technologies, have also been in the billions of dollars. Berkeley Lab was founded in 1931 by Ernest Orlando Lawrence, a UC Berkeley physicist who won the 1939 Nobel Prize in physics for his invention of the cyclotron, a circular particle accelerator that opened the door to high-energy physics. It was Lawrence's belief that scientific research is best done through teams of individuals with different fields of expertise, working together. His teamwork concept is a Berkeley Lab legacy that continues today.