We are seeking a Sr. Research Specialistwhose main responsibility will be to perform sequencing experiments to support epigenetics studies in the Center for Epigenetics using state of the art sequencing platforms. This position will be responsible for executing technical protocols both manually and using automated liquid handling systems as needed. The Sr. Research Specialist must be able to design and execute experiments with independence using knowledge of molecular biology techniques to proactively troubleshoot experiments, resolve technical issues and challenges, and to perform data interpretation. Maintain instruments, contribute to maintaining lab supplies stock and monitor lab freezers. Implement and monitor quality control procedures and consistency across experiments. The position will work with investigators and collaborators to design scientific experiment.
Specific Duties & Responsibilities
Research & Development
Utilize scientific knowledge and experience to conceptualize, design, and advise on research projects with the Division of Molecular Medicine as well as collaborators from other Departments.
Perform and troubleshoot genomic assays independently.
Responsible for co-authorship of manuscripts, and readings of most grants and manuscripts.
Design and oversee multiple extensive databases that integrate clinical, biological and molecular data from the laboratory and from collaborators.
Meet with investigators to help plan experiments from standpoint of correct scientific design.
Keep up to date with new developments in the sequencing and single cell genomics space and import and implement new assays as appropriate.
Construction of various types of libraries for Illumina sequencing. Including libraries for Whole Genome Bisulfite Sequencing (WGBS), RNA-seq, single cell methylation seq, single cell RNA-seq and single cell ATAC-seq
Library preparation for PromethION Oxford Nanopore sequencer.
Operate PromethION sequencer, monitor and review runs.
Perform High molecular weight DNA extractions, regular DNA/RNA extraction and sample quality control.
Perform library quantification via qPCR and Qubit.
Maintain accurate documentation, data records and laboratory notebook.
Follows established molecular biology procedures and protocols accurately and follow best practices guidelines for experiments.
Receiving and unpacking of reagent and supply orders for the laboratory.
Monitor freezers and temperature monitoring system in the East Baltimore and Homewood laboratory facilities. Be available after hours and on weekends to respond to freezer emergencies if needed and ensure the protection of invaluable samples and assets. Contribute to updates and implementation of freezer emergency plans and procedures.
Manage samples, update freezer sample inventory and track samples throughout the freezers.
Interface with co-investigator and collaborators in providing laboratory data.
Maintain chemical reagent inventory.
Contribute to the general maintenance and cleaning of the laboratory, equipment and supplies.
Contribute to keeping the laboratory space compliant with safety regulations and help prepare for annual health and safety inspections.
Bachelor's Degree in Biology, Chemistry or related field.
Four years experience in a comparable laboratory performing relevant techniques.
Master's Degree, with related graduate research, may substitute for experience to the extent permitted by the JHU equivalency formula.
Strong attention to details is critical.
Demonstrates ability for self-direction and initiation, as well as ability to work independently and as part of a team, to address problems/tasks.
Ability to quickly master new laboratory techniques and procedures and tolerance for repetitive tasks.
Must be organized and able to appropriately prioritize responsibilities in a fast-paced environment.
Classified Title: Sr. Research Specialist Role/Level/Range: ACRP/04/MC Starting Salary Range: $46,560 - $64,100 Commensurate with experience Employee group: Full Time Schedule: M-F 8:30-5pm Exempt Status: Exempt Location: School of Medicine Campus Department name: SOM DOM Molecular Medicine Personnel area: School of Medicine
Please refer to the job description above to see which forms of equivalency are permitted for this position. If permitted, equivalencies will follow these guidelines:
30 undergraduate degree credits (semester hours) or 18 graduate degree credits may substitute for one year of experience. Additional related experience may substitute for required education on the same basis. For jobs where equivalency is permitted, up to two years of non-related college course work may be applied towards the total minimum education/experience required for the respective job.
The successful candidate(s) for this position will be subject to a pre-employment background check.
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