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Posted: 26-Aug-22
Location: La Jolla, California
Salary: Open
Internal Number: 118406
UCSD Layoff from Career Appointment: Apply by 08/26/2022 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 09/07/2022. Eligible Special Selection clients should contact their Disability Counselor for assistance.
Effective 9/23: Depending on the experience of the selected candidate, this position will be filled at the SRA 2 or SRA 3 level.
DESCRIPTION
The Department of Medicine is responsible for fulfilling the teaching, research, and clinical missions of the University of California, San Diego and employs 486+ full-time salaried faculty members and 100+ academic appointees. Staff personnel include 600+ staff in 9 bargaining units, students, and volunteers. There are 250 clinical faculty practicing at multiple sites and hospitals.
The overall focus of the laboratory is to understand how epithelial stem cells coordinate tissue renewal with the ultimate goal to harness stem cell regenerative potential to develop new strategies for precise tissue repair. Apply core techniques in cell biology, microscopy, mouse genetics and biophysics to address fundamental questions in stem cell biology during normal development and cancer progression. Under general supervision, the Senior Staff Scientist will independently conduct experiments, make observations, and keep detailed records of all aspects of experimental data related to research projects. Will independently perform molecular biological techniques in the development of plasmid and viral based gene transfer vectors and gene expression analyses (qRT-PCR and RNA-sequencing). Conduct procedures to carry out animal studies including breeding, injections, and maintaining and screening transgenic mouse colony. Perform in vivo fluorescent imaging and histology.
Incumbent will independently perform in vivo and in vitro assays, train new lab members, plan experiments, analyze data, and contribute to scientific papers. Incumbent will assist in data preparation and drafting of manuscripts assisting author or co-authoring scientific articles and some grant writing. Researching journal articles to find appropriate protocols and regulations for experiments. Prepare scientific abstracts and subsequent presentations for relevant scientific meetings as well as for progress reports. Adhere to laboratory environmental health and safety guidelines, including appropriate chemical waste and biological waste disposal.
Incumbent will be responsible for laboratory management including the ordering and maintenance of lab equipment and reagents, monitoring lab expenditures/grant spending, and overseeing the maintenance and organizing of laboratory stocks and ordering supplies, including management of laboratory sample inventories. Incumbent will serve as the laboratory safety officer to ensure that safe work practices are followed as dictated by EH&S guidelines.
MINIMUM QUALIFICATIONS
Theoretical knowledge in cell biology, molecular biology genetics, stem cell biology and developmental biology or equivalent combination of experience and education.
Experience in designing, performing and analyzing research projects and understands what it takes to keep an organized yet fast-paced lab functional.
Experience and knowledge with animal procedures, breeding, handling, anesthesia, injection, transplantation, and colony maintenance as well as the ability to learn in utero-lentivirus injections and intravital fluorescent imaging.
Comfortable of handling and working with mice, primary human tissues and hazardous materials.
Expertise in molecular biology including RNA/DNA isolation, real-time PCR, viral production, plasmid design, cloning and preparation.
Experience with all aspects of tissue culture including freezing, thawing, counting cells and splitting cultures.
Demonstrated experience reading and using Material Safety Data Sheets. Demonstrated ability to follow established Safety and Biohazardous Material Guidelines.
Demonstrated experience working in and maintaining a sterile environment.
Experience with the use of general and special laboratory equipment such as ultracentrifuge, cryostat and microscopes.
Demonstrated skill in accurate record keeping and ability to organize, analyze, and calculate data. Knowledge in basic math for maintaining ledgers, income and expenditure reports.
Ability to keep accurate records of experimental procedures and results, to discuss them, and to prepare relevant oral and written presentations. Ability to present data in a clear and concise manner.
Strong computer skills and experience with the operation of all basic software applications
Connections working at University of California San DiegoUC San Diego is an academic powerhouse and economic engine, recognized as one of the top 8 public universities by U.S. News and World Report. Innovation is central to who we are and what we do. Here, students learn that knowledge isn't just acquired in the classroom - life is their laboratory. UC San Diego's rich academic portfolio includes six undergraduate colleges, five academic divisions and five graduate and professional schools. The university's award-winning scholars are experts at the forefront of their fields with an impressive track record for achieving scientific, medical and technological breakthroughs.
