The Department of OBGYN is seeking to hire a Staff Associate II to study human genetic and molecular basis of human longevity using human stem cell and mouse model within the laboratory of Dr. Yousin Suh. Research approaches include genome editing in pluripotent stem cell (PSC), lineage-committed PSC differentiation and in vivo functional studies in mouse models, and. In addition, the individual will be responsible for lab meeting organization and routine lab maintenance as well as facilitating the day to day cellular and molecular biology laboratory operations.
Job Responsibilities:
Conduct genome editing in PSC using CRISPR/Cas9-mediated gene editing.
Conduct DNA extraction and genotyping.
Conduct RNA and protein extractions.
Conduct bacteria transformation and plasmid prep.
Conduct differentiations and maintenance of somatic cell types including mesenchymal stem cell, endothelial cells and neural stem cell from PSCs
Maintain in vitro cell cultures lines and run established in vitro assays.
Mouse colony maintenance, including but not restricted to breeding set up, weaning, and genotyping.
Conduct behavioral tests, including Open Field, Y maze, and etc.
Conduct non-survival surgeries on mice, such as mouse cardiac perfusion.
Perform qPCR, digital PCR, Western blotting and immunofluorescence staining.
Perform other related duties necessary to the functioning of the laboratory or research project.
Organize lab meetings.
Maintain equipment including CellRaft Air System, cryostat, and etc.
Stock maintenance and order laboratory supplies.
The Scientific Community: Situated on a 20-acre campus in Northern Manhattan CUIMC provides global leadership in scientific research, health and medical education, and patient care. CUIMC is especially proud of its relationship with the surrounding Washington Heights community and has numerous research and clinical programs in Northern Manhattan, including Harlem, Washington Heights, and Inwood. CUIMC also has more than 40 state-of-the-art shared research facilities physically housed in and administered by its departments, centers, and institutes. These resources offer the highest-quality scientific technology to the community, and many also offer education and training, as needed. We offer competitive salary and benefit packages.
The Department of Obstetrics and Gynecology is dedicated to the goal of building a multicultural faculty and staff committed to teaching, working and serving in a diverse community, and strongly encourages applications from candidates of traditionally underrepresented backgrounds.
We are continuously seeking to recruit individuals who will enhance the diversity of our workplace and the effectiveness of our organization.
Minimum Qualification:
Requires a bachelor's degree in biology or related science and minimal 4 years of relevant work experience.
Abundant knowledge and experience in CRISPR/Cas9-mediated gene editing and human pluripotent stem cell technology is needed.
Ability to work with mouse, including mouse handling, ear tagging, injections, and etc.
Ability to provide routine analysis and interpretation of research data
Preferred Qualifications:
Preferred a master's degree in biology or related science
Experience in genome and transcriptome analysis
Experience in mouse behavioral tests
Experience in animal tissue dissections, such as hippocampus dissection, ovary dissection, and etc.
Experience in mouse survival or non-survival surgeries.
Columbia University is an Equal Opportunity Employer / Disability / Veteran
Pay Transparency Disclosure
The salary of the finalist selected for this role will be set based on a variety of factors, including but not limited to departmental budgets, qualifications, experience, education, licenses, specialty, and training. The above hiring range represents the University's good faith and reasonable estimate of the range of possible compensation at the time of posting.
Columbia University is one of the world's most important centers of research and at the same time a distinctive and distinguished learning environment for undergraduates and graduate students in many scholarly and professional fields. The University recognizes the importance of its location in New York City and seeks to link its research and teaching to the vast resources of a great metropolis. It seeks to attract a diverse and international faculty and student body, to support research and teaching on global issues, and to create academic relationships with many countries and regions. It expects all areas of the university to advance knowledge and learning at the highest level and to convey the products of its efforts to the world.