We have an exciting opportunity for a Research Associate I or II to work to develop in vitro assays to study Huntingtonâ™s disease in human pluripotent stem cells. The project requires cell culture experience and coding skills. We are looking for candidates with the desire to learn and work in diverse roles, with over half of the time spend on wet lab experiments and a decent amount of the time on microscopy, data analysis and coding. The person in this position will work with hPSC from patients with Huntingtonâ™s Disease (HD) with the goal to elucidate molecular mechanisms driving these disorders and to develop platforms for drug discovery.
About our group:
We are an academic lab with over 20 BS-, MS-, and PhD-level neuroscientists and engineers that study neurodegenerative diseases such as ALS, Huntingtonâ™s disease, Parkinsonâ™s disease, Alzheimerâ™s disease and other dementias. We use different cellular model systems, including human stem cells that are derived from patients, to answer basic questions in neuroscience as well as discover new therapies for these neurodegenerative diseases.Â At the core of our research are novel longitudinal imaging systems we call robotic microscopes, that we have invented to collect images and time-lapse movies of fluorescent labeled neurons in a high throughput format. We use our microscopy system to extract various types of information about how patient stem cell derived neurons grow, function, degenerate, and respond to candidate drugs. We also take advantage of the terabytes of data that we generate every day and use deep learning to invent new methods for analyzing our images. We have collaborations with many industry and academic groups, publish our work in high profile scientific journals, and present our work at national and international conferences. We continue to design and build novel automated microscopy systems and we need your help to do it.
About the project and team:
Huntingtonâ™s disease is caused by an unstable genetic â˜CAGâ™ sequence that is repetitive and exceeds the normal small amount of repetitions observed in healthy individuals. The person in this position will investigate the role of the instability of this disease-causing repetitive sequence in Huntingtonâ™s disease using neuronal derivatives from hPSC. This person will subject robotic microscopy to human disease hPSC models and use numerous image analysis-based programs to capture phenotypes in a high throughput format, as well as deep learning to determine repeat instability over time.
The person will be part of a team within the Finkbeiner lab that studies the role of DNA damage, repeat instability and epigenetics in neurodegeneration and other neuronal disease phenotypes with robotic microscopy using human disease models and deep learning. The team is diverse, very collaborative and rigorous in the science we produce.
Associates degree and 2+ years relevant experience or
BS in biomedical sciences, biology, bio-engineering or related field is required.
Cell culture experience
Knowledge of one or more of the following languages: Python, Java, C++, Matlab, R
Critical thinking and attention to detail
Highly organized with excellent communication skills
Able to work effectively in small groups
Interest in biology, neuroscience and coding
Human pluripotent stem cell culture experience
Experience with microscopy, especially epifluorescence microscopy
Experience with MicroManager and image analysis software, such as ImageJ
Experience with coding for data analysis problems
Experience with deep learning and feature recognition
Familiarity and interest in neurodegenerative diseases
Hiring range: Â
Research Associate I (non-exempt)- $52K ($25/hr) - $61,177 ($29.41/hr)
The Gladstone Institutes is an independent, not-for-profit research institution affiliated with the University of California San Francisco (UCSF), transforming the health and well-being of all people through medical research, education, and outreach in the fields focused on heart disease, immunology and virology including HIV/AIDS, and neurological disease. Home to notable leaders in the field of medical science, including 2012 Nobel Prize in Physiology or Medicine recipient Shinya Yamanaka, M.D.,Ph.D. Gladstone is comprised of four institutes in addition to various Centers and programs focusing approximately 500 members of our team on its mission: Science Overcoming Disease. Located in an award-winning building adjacent to UCSF's Mission Bay Campus, Gladstone offers a competitive salary and benefits program within a robust environment for those seeking to maximize their potential. Please consider Gladstone and make a difference in human health.