UCSD Layoff from Career Appointment: Apply by 05/26/2022 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 06/07/2022. Eligible Special Selection clients should contact their Disability Counselor for assistance.
An exciting opportunity for a research assistant to join a vibrant, energetic, newly-established laboratory focused on breast cancer metastasis, situated on the UCSD medical school campus.
This position will work closely with the PI, assisting with planning and conducting projects as well as develop and pro-actively manage the laboratory's operational infrastructure. Perfect for highly-motivated individuals seeking a career in cancer research, and who wish to play an instrumental role in developing a new laboratory.
The incumbent will assist in potential projects by employing established breast cancer cell lines and patient-derived xenograft (PDX) models to examine stem-like breast cancer cell survival and 3D clonogenicity in vitro, as well as tumor initiation, recurrence and metastasis in mice +/- delivery of experimental therapeutics. Cutting-edge technologies such as single-cell RNA-Seq, proteomics and fresh patient tissues will also be used in future studies. Experience with these or similar techniques is highly desirable. The incumbent will assist in analyzing RNA and protein levels by QPCR or Western blot respectively, detecting proteins by antibody staining in live or fixed cells and tissues, tissue culture and aseptic technique, modifying gene expression with cDNA, shRNA or CRISPR and assays measuring cell survival and proliferation.
Experience handling, maintaining or injecting mice is preferred. Must possess skills to thrive in a fast-paced and dynamic research environment, including the ability to coordinate and manage multiple tasks simultaneously, work under constant deadlines and practice good time-management. Competitive candidates will be well-organized, detail-oriented and have outstanding communication skills. While primarily focused on research, additional duties include writing and updating protocols, maintaining lab stocks and equipment, as well as ensuring a safe working environment.
Enthusiasm for learning and a genuine interest in scientific research. Willingness and ability to learn new skills, research methods and scientific principles.
Detail-oriented. Ability to work with little supervision, follow verbal and/or written instructions and accurately record data.
Strong communication skills (both verbal and written) needed to interact professionally and effectively in the work environment. Ability to read, comprehend and discuss research materials.
Experienced with aseptic technique and mammalian tissue culture including preparing media, passaging cells with trypsin, and cryo-preserving cells in liquid nitrogen.
Ability and willingness to handle and work with small animals.
Familiar with basic methods of assessing RNA and protein levels, including harvest, calculating concentrations and analyzing by QPCR or Western Blot respectively.
Theoretical knowledge of biochemistry, cell and molecular biology.
Ability to adhere to established UCSD guidelines for safety and environmental issues including proper handling of chemical and biohazard materials.
Good computer skills including competency with Microsoft Word, Excel and PowerPoint.
Familiar with the care and use of basic laboratory equipment such as centrifuges, balances, pH meters, autoclaves and electrophoresis equipment.
Ability to perform antibody staining on cells or fixed tissues to detect proteins by flow cytometry or immunohistochemistry respectively.
Generally familiar with the care and use of microscopes, with specific experience in confocal microscopy to visualize protein localization in cells or tissues.
Experience working with controlled substances, such as anesthetics.
Experience performing minor mouse surgery, including anesthesia, cauterization, suture and wound-clip.
Familiar with different methods of drug administration in mice, such as intraperitoneal injection, oral gavage and intravenous delivery.
Specific experience with basic tumor biology techniques including soft agar clonogenic assays and tumor xenografts in mice.
Experience maintaining a mouse breeding colony.
Familiar with the UCSD online ordering system
Employment is subject to a criminal background check.
Must be willing and able to work with hazardous materials
UC San Diego is an academic powerhouse and economic engine, recognized as one of the top 8 public universities by U.S. News and World Report. Innovation is central to who we are and what we do. Here, students learn that knowledge isn't just acquired in the classroom - life is their laboratory. UC San Diego's rich academic portfolio includes six undergraduate colleges, five academic divisions and five graduate and professional schools. The university's award-winning scholars are experts at the forefront of their fields with an impressive track record for achieving scientific, medical and technological breakthroughs.