Contribute scientifically (molecular biology/computational biology) to help eGenesis deliver safe and effective human transplantable cells, tissues and organs utilizing the latest advancements in genome editing. This Molecular Biologist/Genomic Scientist will be responsible for engineering the first-ever genetically engineered pigs at the functional-genomics level for the purpose of xenotransplantation. Contributions include: a) conducting genome-level structural and chromatin landscape experiments to investigate the effects of trans-gene insertion on native chromosome structure and neighborhood gene regulations, and b) designing and executing gene editing strategies to minimize transgene impact on native chromosome structure and neighborhood gene regulations to achieve optimal stable gene expression for xenotransplantation.
Specific job duties include the following: designing molecular biology/gene-editing experiments to engineer porcine cells; executing wet-bench molecular biology experiments including NGS library preps and sequencing on the Illumina and Nanopore technology platforms; and performing computational data analyses and processing in Linux/AWS.
Must have PhD in Genomics or related field and 3 years of research experience in the field of functional genomics. Experience must include the following areas:
Gene regulation, including cis-regulatory elements mapping, enhancer-promoter looping and other sub-TAD loops, TAD boundary mapping and validation
Computational Biology (Linux, R and Python), especially single cell data (scRNA-seq and scATAC-seq). Integrated analysis of cis-regulatory elements and gene expression. Pathway analysis such as gene set enrichment analysis (GSEA or equivalent)
Performing chromosome architecture assays (Circular Chromosome Conformation Capture (4C-seq)). Library preparation, quality control and data analysis are must-have
Utilizing CRISPR-Cas9 (classical Cas9 double strand break, dCas9-based activation and repression, base editing and HDR are must have), guide RNA design, cloning, transfection, selection and assay read-out, and both on-target and off-target validation
Performing open chromatin mapping assays (ATAC-seq or equivalent). Library preparation, quality control and data analysis are must-have
Transcription factor and histone modification genome-wide mapping (ChIP-Seq, Cut and Tag or equivalent), Library preparation, quality control and data analysis are must-have
Mammalian cell culture (3D organoid culture and iPSC/ES cells are plus), Flow cytometry assay and analysis
Transposon-based gene editing or genomic screening method
Molecular Biology techniques (fragment cloning, site-directed mutagenesis, PCR, Restriction Fragment Length Polymorphism (RFLP), plasmid DNA prep)
Rodent handling, tail vein injection, tail genomic DNA isolation and genotyping by PCR
Protein assays including protein isolation, quantification by Braford assay or equivalent and Western blot
Gene expression assays including RNA isolation and quality control, qRT-PCR or equivalent assays design, operation, and data analysis
Transgene construct assay (dual luciferase reporter assays or equivalent), study design, operation, and data analysis
Must have publication record in peer-reviewed journals in the field of functional genomics, specifically in gene regulation (via enhancer looping and other intra-TAD looping).