- 10% Review literature relating to research projects and assist in writing publications, summary reports, and research grants - 10% Training and supervising undergraduate and pre-doctoral researchers - 10% Perform molecular analyses including PCR, quantitative RT-PCR, DNA extraction, molecular cloning of influenza reverse genetics constructs, CRISPR/Cas9, primer design. - 40% With oversight from PI, design and execute complex, multi-step experiments, trouble-shoot protocols, interpret findings and prepare data for publication in an assigned area to aid in the solution of a research problem. The primary focus of this project will understanding effects of microbiome on influenza susceptibility in a primary bronchial epithelial-respiratory microbiome co-culture model. An experimental approach will be formulated based on existing knowledge of the field, review of relevant literature, contacting other researchers in the field and collaboration with other labs. - 20% Analyze and interpret microbiome and viral infection data from longitudinal observational study -10% Duties as assigned
Independence and decision making: Solves complex research related work assignments with working knowledge of the field and minimal guidance; likely to act as an informal resource for colleagues with less experience
- Ph.D degree within a biomedical or computational biology field. - Experience with standard mammalian tissue culture techniques - Experience with standard molecular biology techniques e.g. PCR, quantitative PCR, DNA extraction - Experience with publication and presentation of data in multiple forms, including manuscripts and grant submission - Experience mentoring trainees including undergraduates, medical students, and/or PhD students in the conduct of research
- Foundational knowledge of microbiology, virology and human immunology - Experience with culture of primary cells - Experience with influenza virus culture and reverse genetics systems - Experience with flow cytometry - Experience with use and/or development of data analysis and/or data visualization tools including R and Python - Working knowledge of “omics” analysis pipelines with specific focus on microbiome data - Strong organizational skills - Strong written and verbal communication skills
The University of Minnesota, founded in the belief that all people are enriched by understanding, is dedicated to the advancement of learning and the search for truth; to the sharing of this knowledge through education for a diverse community; and to the application of this knowledge to benefit the people of the state, the nation, and the world.