The Division of Nephrology seeks a Technician B to assist in executing the aims of funded grants and contracts. The work performed will support genetic investigations of kidney diseases in humans and animal models. The research will have direct relevance to clinical medicine, particularly to the pathophysiology of renal disease in humans. It is expected that this position will provide substantial experience for individuals interested in a career in medicine or biomedical research. In the long term, this position is expected to provide the technician with substantial experience in state-of-the-art approaches in molecular genetics.
Conduct standard molecular, genetic and immunological analysis experiments designed to resolve the genetic basis of kidney disease in human and murine models.
Prepare genomic DNA from human blood or mouse tissue for genotyping.
Perform expression studies using Northern analysis, protein gels with Western blotting and quantitative PCR analyses, ELISA, flow cytometry as well as experiments requiring cell culture.
Analyze DNA and RNA sequence data, and gain exposure to analysis and validation of Next-Gen sequencing data.
Responsible for animal husbandry tasks associated with the genetic studies.
Responsible for setting up matings, weaning pups, tail biopsy, euthanasia and observations on all animals for the development of phenotypes.
Perform data entry and handling all paper work required for the animal facility.
Perform additional related duties as assigned.
Bachelor's degree in chemistry, biology or physics or related science or equivalent combination of education, training and experience
At least 3 months previous laboratory experience preferably with some training either in molecular biological techniques or previous experience handling rodents in the laboratory setting
Must be a team player and be willing to work with human blood, mouse tissue and radionucleotides
Computer skills, especially with Macintosh and Windows computers
The candidate selected for this position is required to undergo a drug screening test prior to a final offer of employment being made.
Participation in the medical surveillance program
Work with laboratory animals
Potential bloodborne pathogen exposure
Equal Opportunity Employer / Disability / Veteran
Columbia University is committed to the hiring of qualified local residents.
Columbia University is one of the world's most important centers of research and at the same time a distinctive and distinguished learning environment for undergraduates and graduate students in many scholarly and professional fields. The University recognizes the importance of its location in New York City and seeks to link its research and teaching to the vast resources of a great metropolis. It seeks to attract a diverse and international faculty and student body, to support research and teaching on global issues, and to create academic relationships with many countries and regions. It expects all areas of the university to advance knowledge and learning at the highest level and to convey the products of its efforts to the world.