The Center for Translational & Computational Neuroimmunology (CTCN), in the Department of Neurology at Columbia University Irving Medical Center (CUIMC) is a hub for large-scale translational human studies that integrate rigorous application of advanced laboratory techniques with cutting-edge computational methods. The CTCN includes a Neuroimmunology Core that is part of the national Alzheimer???s Disease Research Center network in the U.S., and it specializes in the optimization and deployment of novel experimental platforms to support the projects of CTCN investigators and collaborators.
We are currently seeking a Research Assistant to join our research effort in the CNS tissue-based profiling. We are deploying traditional (immunohistochemistry/fluorescence, in situ hybridization) and emerging (spatial transcriptomic,???) technologies coupled to a robust image capture and semi-automated image segmentation pipeline to enable the large-scale projects that are currently funded.
(80%) Cutting-edge single cell technologies
Prepare single cell or single nuclei samples from complex tissues (human or mouse) and generate single cell libraries for multiple applications
Prepare tissues for cryo-sectioning, imaging, and for spatial transcriptome workflow
Evaluate and implement cutting-edge single cell technology workflows
Maintain records, prepare results and present findings
Work closely with laboratory and bioinformatics scientists to create industry-leading single-cell genomics data and knowledge
Learn new protocols as new technologies are introduced
(20%) Biobank responsibilities
Provide assistance with laboratory processing and shipment of biospecimen (e.g. blood, serum, plasma, DNA, CSF) per research protocol.
Responsible for entering data into the appropriate database; quality assurance activities as assigned to ensure that study data are entered and transmitted in a timely fashion.
Bachelor's degree and at least one and one-half years of related experience or equivalent in education, training, and experience.
Knowledge of various related laboratory procedures and techniques.
Bachelor???s degree in a life science discipline, with at least 3 months to one year of full-time molecular biology experience in an academic or industrial setting
Basic understanding of molecular biology and analytical techniques including PCR, RT-qPCR, molecular cloning, nucleic acid isolation and manipulation.
Ability to learn new skills and knowledge on the job and productively deliver high-quality results
Attention to detail and maintenance of good scientific records
Experience working with RNA (bulk and single-cell methods) is strongly preferred
Experience handling human clinical biopsies or animal tissues is preferred
Work effectively in a team environment.
Excellent communication and organization skills as well as the ability to work independently.
Equal Opportunity Employer / Disability / Veteran
Columbia University is committed to the hiring of qualified local residents.
Columbia University is one of the world's most important centers of research and at the same time a distinctive and distinguished learning environment for undergraduates and graduate students in many scholarly and professional fields. The University recognizes the importance of its location in New York City and seeks to link its research and teaching to the vast resources of a great metropolis. It seeks to attract a diverse and international faculty and student body, to support research and teaching on global issues, and to create academic relationships with many countries and regions. It expects all areas of the university to advance knowledge and learning at the highest level and to convey the products of its efforts to the world.