PostDoctoral Associate in Biophysics and Structural Biology
Location: New Brunswick, New Jersey
Internal Number: 125150
A postdoctoral position in cryo-electron microscopy (cryo-EM) and single-molecule studies of DNA repair and replication is available in the laboratory of Dr. Arek Kulczyk in the Institute for Quantitative Biomedicine at Rutgers University.
Kulczyk lab (http://kulczyk-lab.cryoemcorp.com) integrates structural approaches, in particular single-particle cryo-EM, single-molecule methods and novel correlative light and electron microscopy (CLEM) techniques to study DNA replication and repair (Kulczyk et al., 2017, PNAS, 114:1848; Duderstadt et. al., 2016, Mol Cell, 64:1035; Geertsema et al., 2014, PNAS, 111:4073; Kulczyk et al., 2012, PNAS, 109:9408; Loparo et al., 2011, PNAS, 108:3584).
Minimum Education and Experience:
Applicants must hold a PhD in biophysics or in a related field and have a deep working knowledge of structural biology.
Required Knowledge, Skills, and Abilities:
Proficiency in cloning, protein expression, protein purification, and characterization of protein-nucleic acid complexes using a variety of biophysical methods (for example: BIAcore, ITC, AUC, DLS and NMR). Research experience in cryo-electron microscopy (cryo-EM) and single-molecule biophysics is desirable, however not required. The selected candidate will obtain appropriate training in microscope alignments, data acquisition, image processing and structure calculation using a variety of cryo-EM software. The candidate should be proficient in Linux. Working knowledge of C++, Python, CUDA and experience in high performance computing (HPC) is desirable, but not required. Strong written and oral communication skills in English, as well as the ability to work independently and prepare scientific manuscripts are required.
The Institute for Quantitative Biomedicine houses the state-of-the-art cryo-EM facility (Thermo Fisher Scientific Talos Arctica cryo-electron microscope equipped with a Summit K2 direct electron detector, BioQuantum energy filter and a Volta phase plate; Leica DM6 FS/EM cryo-CLEM system), NMR facility (Bruker 800 MHz and 700 MHz with cryoprobes and two 600 MHz spectrometers), mass spectrometry and super-resolution imaging facilities, and is the world headquarters of the Research Collaboratory for Structural Bioinformatics (RCSB) consortium and the Protein Data Bank (PDB). In addition, the effort to purchase Thermo Fisher Scientific Titan Krios and dual-beam FIB-SEM instruments is under way.
Rutgers, The State University of New Jersey, is a leading national public research university and the state's preeminent, comprehensive public institution of higher education. Rutgers is dedicated to teaching that meets the highest standards of excellence; to conducting research that breaks new ground; and to turning knowledge into solutions for local, national, and global communities. As it was at our founding in 1766, the heart of our mission is preparing students to become productive members of society and good citizens of the world. Rutgers teaches across the full educational spectrum: preschool to precollege; undergraduate to graduate and postdoctoral; and continuing education for professional and personal advancement. Rutgers is New Jersey's land-grant institution and one of the nation's foremost research universities, and as such, we educate, make discoveries, serve as an engine of economic growth, and generate ideas for improving people's lives.