The Brain Image Library (BIL), is the NIH-designated microscopy archive for the BRAIN Initiative. The data scope of the BIL includes whole-brain microscopy image datasets and their accompanying secondary data such as neuron morphologies, targeted microscope-enabled experiments including connectivity between cells, spatial transcriptomics, and historical collections of value to the community. The collection currently contains thousands of mouse brain datasets, and to a lesser extent, datasets from primates. The technologies most frequently used to generate the data currently deposited in the library include serial two-photon tomography (STPT), fluorescence micro-optical sectioning tomography (fMOST), oblique light-sheet tomography (OLST) and others. Carnegie Mellon University’s Pi
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