This is a research assistant I position in Prof. George Church's group, where you will be working with a senior postdoc in the field of Genetic code Expansion.
This is a good opportunity if you are interested in synthetic biology & chemical biology projects, with a potential for altering one of the oldest languages (billion year) in the world, the language of the triplet genetic codon that dictates how proteins are made by all living cells. Proteins with facile and augmented chemical properties can be generated by genetic incorporation of non-standard amino acids (nsAA), beyond the lexicon of the 20 standard amino acids. The nsAAs can be used to enhance catalytic activity of enzymes, build augmented materials and therapeutics. You will have the opportunities to work with senior researchers on several aspects of expanding the genetic code i.e generating Ribosome variants, engineering E.coli strains, developing directed evolution pipelines, and engineering orthogonal enzymes.
Specific responsibilities will include in-silico modeling of enzymes using structural approaches (Rosetta) and using rational protein engineering to construct libraries for high-throughput screening. Additionally, tasks will involve streamlining analyses of large NGS datasets from the screens to discover statistically enriched patterns. Interesting hits from the screens will be pursued for further experimental characterization through a range of plate-reader assays, LC-MS, or other in-vitro bio-assays. The RA should uphold Good Laboratory Practices and diligently document experiments and data in both physical and electronic laboratory notebooks.
What we offer: 1. You will have the opportunity to work with senior lab members, participate in lab group meetings and network with a number of scientific leaders that could help in career development. 2. Candidates with an entrepreneurial attitude will be viewed positively and you will have the opportunity to potentially get involved in venture creation with the senior researchers during the course of the project. 3. Applications from historically underrepresented groups and minorities will be considered favorably.
This is a one-year term position, fully benefits eligible, with possibility of extension contingent on work performance and continued funding to support the position.
Harvard requires COVID vaccination for all Harvard community members. Individuals may claim exemption from the vaccine requirement for medical or religious reasons. More information regarding the University's COVID vaccination requirement, exemptions, and verification of vaccination status may be found at the University's
At least one year related work experience (relevant course work may count towards experience).
Bachelor's or Master's degree preferred. 1. While students with a background in one of the following: Chemical Engineering, Chemical Biology, Bioinformatics, Systems Biology Biochemistry & Molecular Biology will suit the demands of the project, we welcome applications from diverse quantitative training backgrounds (eg; Physics, Engineering, Computer science) who would like to work in an interdisciplinary setting. Students with working experience in any laboratory setting of at least six months will be viewed favorably. 2. Experience in protein library design, genetic or metabolic engineering and next-gen sequencing analysis will be viewed favorably. 3. Experience working in a startup or industry will be an advantage. 4. Strong verbal and written communication skills. Experience with coding (eg; Python, R) is essential.
EQUAL OPPORTUNITY EMPLOYER: We are an equal opportunity employer and all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability status, protected veteran status, or any other characteristic protected by law.
The Edmond J. Safra Center for Ethics at Harvard University seeks to strengthen teaching and research about pressing ethical issues; to foster sound norms of ethical reasoning and civic discussion; and to share the work of our community in the public interest.
Widespread ethical lapses of leaders in government, business and other professions prompt demands for more and better moral education. More fundamentally, the increasing complexity of public life - the scale and range of problems and the variety of knowledge required to deal with them - make ethical issues more difficult, even for men and women of good moral character. Not only are the ethical issues we face more complex, but the people we face them with are more diverse, increasing the frequency and intensity of our ethical disagreements.
Given these changes in the United States and in societies around the globe, the Center seeks to help meet the growing need for teachers, scholars, and leaders who address questions of moral choice across many of the professions and in public life more generally, and promotes a perspective on ethics informed by both theory and practice. We explore the connection between the problems t...hat professionals confront and the social and political structures in which they act. More generally, we address the ethical issues that all citizens face as they make the choices that profoundly affect the present and future of their societies in our increasingly interdependent world.