UCSD Layoff from Career Appointment: Apply by 12/22/2021 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 01/03/2021. Eligible Special Selection clients should contact their Disability Counselor for assistance.
This posting shall remain open until filled.
The Center for Neural Repair in the UC San Diego Department of Neurosciences studies gene and cell therapies for spinal cord injury and for neurodegenerative disorders such as Alzheimer's disease.
Under supervision and independently, incumbent will provide surgical and veterinary support in a neurosciences research laboratory studying gene therapy in animal models of neurodegenerative disease. Primary responsibilities include rodent surgery, post-operative care, perfusion, and dissection, as well as maintaining transgenic mouse lines. Additional responsibilities may include histology (tissue sectioning, immunohistochemical staining, and microscopy), molecular biology (preparation and validation of plasmid DNA, restriction digest, gel electrophoresis, western blot, ELISA, and qPCR), and cell biology (subculture of immortalized cell lines, live imaging of cultured cells, and immunocytochemistry). Will maintain detailed records, perform rigorous data management, and collaborate effectively with a diverse laboratory staff.
Theoretical knowledge in biology or related field, as typically attained by a Bachelor's degree and/or an equivalent combination of education and experience.
Ability and knowledge in handling and performing survival surgery in rodents.
Ability and knowledge performing rodent perfusion and dissection.
Experience with transgenic mouse breeding and genotyping.
General familiarity with tissue sectioning and immunohistochemistry.
General familiarity with cell and molecular biology techniques.
Strong computer skills and experience with word processing, spreadsheets, and graphics software. Ability to learn new software for analysis of microscopic images.
Excellent verbal, written, and electronic communication skills and ability to keep accurate records, prepare study reports, and write detailed protocols.
Knowledge of basic lab safety requirements and procedures including EH&S regulations and methods for handling, clean-up, and waste disposal for biohazardous materials.
Experience performing stereotactic injection of rodent brain.
Experience performing intrathecal infusion in rodents.
Experience performing light, fluorescent, and confocal microscopy.
Experience performing specific molecular biology techniques including: bacterial culture; plasmid DNA subcloning, verification, and purification; western blot; ELISA; PCR; qPCR; RT-qPCR.
Experience performing specific cell biology techniques including: subculture of immortalized cell lines; electroporation and transfection of cultured cells; live imaging; immunocytochemistry.
Employment is subject to a criminal background check.
Must be willing and able to work with live rats and mice in a research related environment.
Must be willing to work on occasional weekends and holidays.
UC San Diego is an academic powerhouse and economic engine, recognized as one of the top 8 public universities by U.S. News and World Report. Innovation is central to who we are and what we do. Here, students learn that knowledge isn't just acquired in the classroom - life is their laboratory. UC San Diego's rich academic portfolio includes six undergraduate colleges, five academic divisions and five graduate and professional schools. The university's award-winning scholars are experts at the forefront of their fields with an impressive track record for achieving scientific, medical and technological breakthroughs.