Research Associate-Transgenic Mouse Support/Mammalian Gene Expression
Lawrence Berkeley National Laboratory
Internal Number: 94004
Lawrence Berkeley National Lab's (LBNL) Environmental Genomics & Systems Biology Division has an opening for a Research Associate-Transgenic Mouse Support/Mammalian Gene Expression to join the team.
In this exciting role, you will be under direct supervision, where you will work in the Mammalian Functional Genomics Lab to carry out various semi-routine duties related to support of the transgenic mouse group and support of the molecular biology lab as part of several projects aimed at understanding how mammalian gene expression is regulated. You will work with live mice, mouse tissue samples, and perform animal microdissection. You will be expected to assist with analyzing experimental results, generate reports, and present findings at group meetings.
What You Will Do:
Provide support to the transgenic mouse facility, including but not limited to general animal husbandry, intraperitoneal injections of hormones, embryo production, egg collection, microdissection, identification of pseudo-pregnant recipient females, maintenance of mouse colonies, genomic DNA isolation for genotyping of transgenic lines, and euthanasia.
Provide general semi-routine support to molecular biology labs including but not limited to dishwashing, autoclaving of glassware, routine maintenance of lab equipment, buffer production, and supply inventory.
Perform various molecular biology techniques such as PCR, small- and large-scale plasmid preps, restriction enzyme digest, and gel electrophoresis.
Perform minimal analysis with experimental results, generate reports, and present findings.
Maintain careful records of all experiments performed.
Meet regularly with supervisor and other team members to discuss project progress and provide recommendations to improve the experimental pipeline.
Job requires occasional weekend work.
Additional Responsibilities as needed:
Assist with collection of mouse embryonic and adult tissues.
Maintain mouse lineages (mate, wean, tag, ear, or tail tip biopsy).
Molecular cloning tasks including vector ligation and bacterial work (transformation, plating and colony screens).
What is Required:
B.A or B.S degree in Biology or related discipline or equivalent experience.
Willingness to work with mice.
Ability to learn new laboratory protocols and technologies.
Detail-oriented with strong organizational, writing, and record-keeping skills.
Good analytical skills to effectively evaluate data and make recommendations.
Excellent and effective interpersonal communication and presentation skills; ability to interact collaboratively with staff at all levels of the organization.
Must demonstrate efficient time management skills and the ability to multitask.
Competent in the use of computers and related software programs (i.e., PC, Mac, Google Drive).
Willingness to work in a research lab environment with changing priorities and technologies.
Preferred 1+ years of research laboratory and/or research animal experience.
Experience with mouse animal husbandry and/or handling live rodents in a research environment.
Experience with molecular biology techniques: PCR, bacterial transformation, small- and large-scale DNA purification, restriction digest, and gel electrophoresis.
This position is represented by a union for collective bargaining purposes.
This is a full time, 1 year term appointment with the possibility of extension or conversion to Career appointment based upon satisfactory job performance, continuing availability of funds and ongoing operational needs.
This position is represented by a union for collective bargaining purposes.
This position may be subject to a background check. Any convictions will be evaluated to determine if they directly relate to the responsibilities and requirements of the position. Having a conviction history will not automatically disqualify an applicant from being considered for employment.
Work will be performed 5 days a week, with occasional weekend work, at Lawrence Berkeley National Lab, 1 Cyclotron Road, Berkeley, CA.
Equal Employment Opportunity:
Based on University of California Policy - SARS-CoV-2 (COVID-19) Vaccination Program and U.S Federal Government requirements, Berkeley Lab requires that all members of our community obtain the COVID-19 vaccine as soon as they are eligible. As a condition of employment at Berkeley Lab, all Covered Individuals must Participate in the COVID-19 Vaccination Program by providing proof of Full Vaccination or submitting a request for Exception or Deferral. Visit covid.lbl.gov for more information.
Berkeley Lab is committed to Inclusion, Diversity, Equity and Accountability (IDEA) and strives to continue building community with these shared values and commitments. Berkeley Lab is an Equal Opportunity and Affirmative Action Employer. We heartily welcome applications from women, minorities, veterans, and all who would contribute to the Lab's mission of leading scientific discovery, inclusion, and professionalism. In support of our diverse global community, all qualified applicants will be considered for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, or protected veteran status.
In the world of science, Lawrence Berkeley National Laboratory (Berkeley Lab) is synonymous with excellence. Thirteen scientists associated with Berkeley Lab have won the Nobel Prize. Fifty-seven Lab scientists are members of the National Academy of Sciences (NAS), one of the highest honors for a scientist in the United States. Thirteen of our scientists have won the National Medal of Science, our nation's highest award for lifetime achievement in fields of scientific research. Eighteen of our engineers have been elected to the National Academy of Engineering, and three of our scientists have been elected into the Institute of Medicine. In addition, Berkeley Lab has trained thousands of university science and engineering students who are advancing technological innovations across the nation and around the world. Berkeley Lab is a member of the national laboratory system supported by the U.S. Department of Energy through its Office of Science. It is managed by the University of California (UC) and is charged with conducting unclassified research across a wide range of scientific disciplines. Located on a 200-acre site in the hills above the UC Berkeley campus that offers spectacular... views of the San Francisco Bay, Berkeley Lab employs approximately 4,200 scientists, engineers, support staff and students. Its budget for 2011 is $735 million, with an additional $101 million in funding from the American Recovery and Reinvestment Act, for a total of $836 million. A recent study estimates the Laboratory's overall economic impact through direct, indirect and induced spending on the nine counties that make up the San Francisco Bay Area to be nearly $700 million annually. The Lab was also responsible for creating 5,600 jobs locally and 12,000 nationally. The overall economic impact on the national economy is estimated at $1.6 billion a year. Technologies developed at Berkeley Lab have generated billions of dollars in revenues, and thousands of jobs. Savings as a result of Berkeley Lab developments in lighting and windows, and other energy-efficient technologies, have also been in the billions of dollars. Berkeley Lab was founded in 1931 by Ernest Orlando Lawrence, a UC Berkeley physicist who won the 1939 Nobel Prize in physics for his invention of the cyclotron, a circular particle accelerator that opened the door to high-energy physics. It was Lawrence's belief that scientific research is best done through teams of individuals with different fields of expertise, working together. His teamwork concept is a Berkeley Lab legacy that continues today.