The Voskuhl laboratory in the Department of Neurology at UCLA, is recruiting a highly motivated Specialist with expertise in creation of conditional cell-specific gene knockout/knockin mice, the use of RiboTag mice to determine cell-specific gene expression in brain and spinal cord, as well as clinical and neuropathologic evaluations of mice with models of multiple sclerosis (MS), aging, and Alzheimer's Disease.
The Specialist will serve as a principal researcher for creation of models of multiple sclerosis, aging, and Alzheimer's Disease. These diseases will be set up in mice that enable the use of RiboTag technology. A strong working knowledge of genetic engineering of mice with cell-specific promoter driven Cre expression in various brain cells is required, as well as immunoprecipitation of HA-labelled RNAs to define the translatome in each targeted cell of each brain region during disease using RNA sequencing (in collaboration with the sequencing core). Validation of key gene expression changes will be needed at the RNA level using qPCR and at the protein level using immunohistochemistry. Causal linkage of gene expression to its effect on disease will entail the design and creation of mice with cell-specific deletion of the gene of interest identified using RNA sequencing. Whether gene deletion abrogates disease at the clinical and neuropathologic level will be determined through targeted behavioral testing and immunohistochemistry of tissue sections of mice that are a conditional knock out (CKO) vs wild type (WT). Laboratory bench skills including hand-on experience with tissue region-specific isolations (hippocampus, spinal cord, cerebral cortex, cerebellum), tissue sectioning, immunohistochemistry, HA-labelled RNA immunoprecipitation, breeding strategies to create CKO and WT mice, genotyping, qPCR, disease induction of the MS model, EAE clinical scoring, cognitive testing of aging and AD mice in the behavioral core, and the ability to readily use excel, graphics, and statistical analysis software. He/she/they will also serve as a technical consultant for others doing similar work in the lab and contribute to laboratory management tasks, including ordering management of laboratory compliance, ordering laboratory consumables and informative genetically modified mice, and designing breeding strategies between two lines to create novel informative mice.
Appointees in the Specialist series will be expected to engage in specialized research, professional activities and do not have teaching responsibilities. Specialists are expected to use their professional expertise to make scientific and scholarly contributions, and may participate in University and Public Service.
Minimum qualifications: B.S. in molecular biology and at least 10 years of previous research experience. Demonstrated expertise in genetic engineering of mouse strains, animal models of MS, immunohistochemistry, and behavioral testing of mice.
The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age or protected veteran status. For the complete University of California nondiscrimination and affirmative action policy, see: UC Nondiscrimination & Affirmative Action Policy, https://policy.ucop.edu/doc/4000376/DiscHarassAffirmAction
UCLA is known worldwide for the breadth and quality of its academic, research, health care, cultural, continuing education and athletic programs. UCLA offers undergraduate degrees in more than 127 majors and graduate degrees in 198 program areas. UCLA has 11 highly regarded professional schools. Eight are ranked among the nation's top 15 in their field by U.S. News & World Report. UCLA is consistently among the most popular campus in the nation for undergraduate applicants. The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to age, race, color, religion, sex, national origin, disability, or protected veteran status. Benefits:UCLA offers a comprehensive benefits package, including an average of three weeks' vacation per year; an average of 12 days per year sick leave; 13 paid holidays per year; health, dental and optical benefits; life insurance; disability insurance; the University of California Retirement Plan with 5 year vesting and various voluntary UC Savings Plans. There are also special programs and privileges available, such as accessibility to cultural and recreational programs, athletic events, and the University Credit Union.