Our research focus on investigating the role of non-coding RNA in polycystic kidney disease (PKD). We utilize multiple mouse model of the disease to investigate how non-coding RNA affect formation and progression of PKD. Our work include generating mouse models of PKD and performing molecular and physiological analysis to determine the contribution of non-coding RNA in PKD.
Job Duties/Responsibilities: 70% Conducting experiments in collaboration with lab staff • Perform complex assays such as Cross-linking RNA immunoprecipitation (ChIRP), as well novel procedures that include gene manipulation in kidney organoid. • Ability to work with viruses, learn and implement latest gene editing technologies. • Perform DNA cloning to generate plasmids that are utilized in vivo gene expression analysis. • Perform basic mouse surgeries that include kidney perfusion, and organ dissecting. • Utilize software such as Image J to perform complex statistical analysis and measurements such as kidney cyst index and number.
20% Maintenance and Breeding of Transgenic Mice: • Weaning, sexing and breeding of mice • Timely and accurate genotyping and record keeping of mice produced for the lab • Collection of tissue, blood and urine from animal models of kidney disease
10% Lab maintenance duties • Ordering and stocking of lab supplies • Troubleshooting and working with vendors to maintain equipment • Cleaning and organization of lab space
Required Qualifications (Must be documented on application materials): • BS in in biological sciences, preferably genetics and/or molecular biology focused and 2 years of related experience or a combination of education and work experience to equal 6 years • Strong interpersonal communication and organizational skills • Strong understanding of PCR design and troubleshooting • Familiarity with transgenic animal models (Cre/lox and reporter systems in mice) • Willingness to work with animal models of disease and tissue harvest, preparation and genotyping
Preferred Qualifications: • 2 year of related experience working in a laboratory setting • Experience with molecular biology techniques such as qPCR, cloning, CRISPR/Cas9 gene editing, RNA pulldown and In situ hybridization assays • Experience in working with mice • Previous exposure to immunohistochemistry and immunofluorescence microscopy • Ability to perform, design and troubleshoot complex molecular biology protocols • Ability to perform, or willingness to learn, quantification of microscopic imaging • Willingness to have data produced presented to and critiqued by colleagues • Experience working with IACUC and other protocol submissions
Internal Number: 341675
About University of Minnesota, Twin Cities
The University of Minnesota, founded in the belief that all people are enriched by understanding, is dedicated to the advancement of learning and the search for truth; to the sharing of this knowledge through education for a diverse community; and to the application of this knowledge to benefit the people of the state, the nation, and the world.