Caltech is a world-renowned science and engineering institute that marshals some of the world's brightest minds and most innovative tools to address fundamental scientific questions. We thrive on finding and cultivating talented people who are passionate about what they do. Join us and be a part of the diverse Caltech community.
We are seeking a highly motivated and dependable individual to join our integrative computational and experimental team in characterizing how nuclear architecture/influences gene expression and functional outcomes at the level of single cells. Specifically, this person will help with cellular and molecular biology tasks associated with setting up single cells assays for our newly developed SPRITE (Split pool Recognition of Interactions by Tag Extension) method that measures nuclear architecture. These tasks will include conducting experiments using already established SPRITE protocols but also help developing new protocols based on Split-Pool technique. Moreover, the successful candidate will assist with imaging studies that serve to confirm and extend findings resulting from SPRITE experiments. These studies will include helping to prepare RNA- and/or DNA-FISH (Fluorescence In Situ Hybridization) samples in combination with immunofluorescence staining, acquiring images on state-of-the-art microscopes, and assisting with image processing and analysis.
Training in established techniques will be provided as needed, but the work will require significant independence of day-to-day operations to achieve the overall goals of the project.
With close guidance from senior members of the lab and in accordance with guidelines set forth for a particular task, the successful candidate will design and independently perform bench level experiments and small projects that support the overall research objective of an integrative computational and experimental lab in a fast paced and dynamic environment
Job tasks will include, but are not limited to:
Cell culture tasks: maintaining mammalian cells including media and reagent preparation, crosslinking cells to fix DNA-RNA-protein interactions.
Molecular biology tasks: carrying out multiple rounds of split-pool oligo barcode ligation, preparation of a library for sequencing.
Lab support tasks: reagent aliquoting and organization, buffer preparation, helping with inventory of reagents and supplies.
The candidate will interact directly with senior members of the research team and lab head to ensure proper experimental design, analysis, and technical concerns related to the project. The candidate will adapt easily to changing needs and priorities, facilitate project completion, provide lab support and organizational needs, address technical problems, and summarize and present results. Training will be provided as needed.
Bachelor's degree in biology or related field required.
1-2 years of experience in a research laboratory (can include thesis work), preferably in an independent project.
We are an equal opportunity employer and all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability status, protected veteran status, or any other characteristic protected by law.
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The California Institute of Technology (Caltech) is a world-renowned science and engineering research and education institution, where extraordinary faculty and students seek answers to complex questions, discover new knowledge, lead innovation, and transform our future.