The Research Associate will support scientists and senior research associates involved in genetic engineering, functional genomics, control of gene expression, studies of heterochromatin, characterization of cDNAs, sequencing and high-throughput production of proteomics resources. The Research Associate will perform semi-routine research assignments including molecular biological procedures, data collection and processing of data along with minimal analysis, and sample preparation.
What You Will Do:
Genetically engineer and validate the genomes of microorganisms.
Isolate DNA using commercial and non-commercial protocols and kits.
Perform high-throughput DNA sequencing related protocols including, but not limited to, sequencing reaction setup, reaction cleanup, and sample loading on automated DNA analyzers.
Isolate novel cDNA clones through multiple PCR-based strategies.
Query databases to identify targets for capture, sequencing, mutagenesis, and cloning.
Perform general molecular biological and microbiological protocols.
Use recombination-based strategies, including Gateway(tm)and Cre-Lox methods, to move cloned ORF's into a variety of donor and expression vectors.
Minimal computational analysis of sequence data using industry-standard analysis tools.
Analyze results and troubleshoot problems to ensure uninterrupted and reliable production.
Maintain accurate notebook of experiments performed.
Update production databases in a timely fashion.
Conduct routine housekeeping and restock materials in laboratory areas on an as needed basis.
Maintain work area in compliance with LBNL environmental, health and safety regulations.
Maintain current knowledge of genomics by attending the group journal club.
Report progress and experimental results at weekly group meetings.
Maintain and organize frozen clone libraries, including preparing shipments for collaborating laboratories.
What is Required:
Bachelor's degree in Molecular Biology or related field preferred and up to a few years related experience.
Experience in high-throughput molecular biology techniques including DNA sequencing, PCR, DNA isolation and preparation, gel electrophoresis, transformations, cloning, and restriction enzyme analysis.
Careful data and sample management skills.
Good organizational skills.
Ability to work as a team member, as well as individually.
Experience operating an ABI 3730xl DNA analyzer.
Experience with Gateway(tm) cloning.
Experience working in 96-well plate format and use of 8- and 12-channel pipettors.
Experience working in a Biological Safety Cabinet.
Familiarity with UNIX, Windows, and Macintosh operating systems.
Structured Query Languages (SQL).
Ability to multi-task.
This is a full time, term appointment with a hard end date of 5/31/2022. There is the possibility of extension or conversion to Career appointment based upon satisfactory job performance, continuing availability of funds and ongoing operational needs.
This position may be subject to a background check. Any convictions will be evaluated to determine if they directly relate to the responsibilities and requirements of the position. Having a conviction history will not automatically disqualify an applicant from being considered for employment.
Diversity, equity, and inclusion are core values at Berkeley Lab. Our excellence can only be fully realized by faculty, students, and staff who share our commitment to these values. Successful candidates for our faculty positions will demonstrate evidence of a commitment to advancing equity and inclusion.
Work will be primarily performed at: West Berkeley Biocenter (Potter St.) -- Bldg. 977, 717 Potter St., Berkeley, CA.
Learn about us!
Biological Systems and Engineering Division
Vision: To lead efforts that combine the power of biology with the tools of engineering to develop sustainable energy and biomanufacturing solutions and improve human health.
Mission:Advance a mechanistic and predictive understanding of complex biological systems over multiple scales in terms of their responses to manipulation, stress, disease and environmental challenges and translate this knowledge using engineering principles to develop resilient systems, tools, and processes for the efficient production of fuels, chemicals, materials, tissues, and therapeutics.
Working at Berkeley Lab has many rewards including a competitive compensation program, excellent health and welfare programs, a retirement program that is second to none, and outstanding development opportunities. To view information about the many rewards that are offered at Berkeley Lab- Click Here.
Equal Employment Opportunity: Berkeley Lab is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, or protected veteran status. Berkeley Lab is in compliance with the Pay Transparency Nondiscrimination Provision under 41 CFR 60-1.4. Click here to view the poster and supplement: "Equal Employment Opportunity is the Law.
Lawrence Berkeley National Laboratory encourages applications from women, minorities, veterans, and other underrepresented groups presently considering scientific research careers.
Internal Number: 92977
About Lawrence Berkeley National Laboratory
In the world of science, Lawrence Berkeley National Laboratory (Berkeley Lab) is synonymous with excellence. Thirteen scientists associated with Berkeley Lab have won the Nobel Prize. Fifty-seven Lab scientists are members of the National Academy of Sciences (NAS), one of the highest honors for a scientist in the United States. Thirteen of our scientists have won the National Medal of Science, our nation's highest award for lifetime achievement in fields of scientific research. Eighteen of our engineers have been elected to the National Academy of Engineering, and three of our scientists have been elected into the Institute of Medicine. In addition, Berkeley Lab has trained thousands of university science and engineering students who are advancing technological innovations across the nation and around the world. Berkeley Lab is a member of the national laboratory system supported by the U.S. Department of Energy through its Office of Science. It is managed by the University of California (UC) and is charged with conducting unclassified research across a wide range of scientific disciplines. Located on a 200-acre site in the hills above the UC Berkeley campus that offers spectacular... views of the San Francisco Bay, Berkeley Lab employs approximately 4,200 scientists, engineers, support staff and students. Its budget for 2011 is $735 million, with an additional $101 million in funding from the American Recovery and Reinvestment Act, for a total of $836 million. A recent study estimates the Laboratory's overall economic impact through direct, indirect and induced spending on the nine counties that make up the San Francisco Bay Area to be nearly $700 million annually. The Lab was also responsible for creating 5,600 jobs locally and 12,000 nationally. The overall economic impact on the national economy is estimated at $1.6 billion a year. Technologies developed at Berkeley Lab have generated billions of dollars in revenues, and thousands of jobs. Savings as a result of Berkeley Lab developments in lighting and windows, and other energy-efficient technologies, have also been in the billions of dollars. Berkeley Lab was founded in 1931 by Ernest Orlando Lawrence, a UC Berkeley physicist who won the 1939 Nobel Prize in physics for his invention of the cyclotron, a circular particle accelerator that opened the door to high-energy physics. It was Lawrence's belief that scientific research is best done through teams of individuals with different fields of expertise, working together. His teamwork concept is a Berkeley Lab legacy that continues today.