The Wickman laboratory and the Viral Vector and Cloning Core (VVCC) seek a motivated individual to contribute to our missions of generate high-quality custom DNA and viral tools (VVCC) and applying these tools for scientific discovery (Wickman lab).
Primary duties will include:
50% contribution to VVCC in the form of generating and validating custom DNA reagent for VVCC clients, and conducting advanced characterization of AAV and lentiviral vectors that are produced by the VVCC. General support to the VVCC is also expected, including protocol development and optimization, ordering supplies and reagents, ensuring adherence with local regulations guiding work with DNA and viral tools, and maintaining a clean and safe lab environment.
50% contribution to ongoing research projects in the Wickman lab centered on the development and application of custom viral genetic tools and animal models to understanding molecular influences on cell excitability and the cellular/circuit mechanisms underlying behavior. Work will involve the generation and validation of custom DNA reagents and viral vectors, in vitro and in vivo application of these tools, and coordination with local service cores to evaluate the impact of manipulations on gene expression patterns, epigenetic alterations, post-translational modifications of proteins, and neuronal connectivity. General support to the Wickman lab is also expected, including protocol development and optimization, ordering supplies and reagents, ensuring adherence with local regulations guiding work with DNA and viral tools, and maintaining a clean and safe lab environment.
Required Qualifications (must be mentioned on application/resume):
BA/BS in the Life Sciences (e.g., Molecular Biology, Genetics, Biochemistry, Biology, Chemistry) or equivalent combination of education and work experience to equal four years
Experience with techniques in molecular biology, including sub-cloning, PCR, DNA purification, and mutagenesis
Experience with techniques in cell culture (cell lines and/or primary cultures), including maintenance, transfection, etc.
Must be able to lift objects in the lab that are up to 50 pounds (plate boxes, centrifuge rotors, etc.)
One year of experience working in a biomedical research laboratory
Demonstrated proficiency with techniques in molecular biology (e.g., sub-cloning, PCR, qPCR, DNA purification, mutagenesis, DNA sequencing); developing, implementing, and trouble-shooting sub-cloning strategies
Demonstrated proficiency with DNA sequence analysis and the use of related software
Experience with research in mouse models (handling and behavior)
Excellent communication skills, attention to detail, and comfort in working on a team within a fast-paced environment focused on performance and productivity
Internal Number: 341277
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