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The Moffitt laboratory leverages a cutting-edge spatial transcriptomics technique--MERFISH--capable of imaging and identifying thousands of different RNA molecules in their native tissue context. This approach, co-pioneered by Dr. Moffitt, combines the power of single-cell RNA sequencing with high resolution microscopy. With the ability to directly visualize gene expression at the genome-scale within intact tissues slices, it is now possible to create molecularly defined atlases of tissues. And these atlases, in turn, provided the ability to systematically discover, functionally characterize, and map cell types, states, and interactions within a very broad range of tissues across all domains of life.
The Moffitt laboratory is extending this technology by increasing its imaging throughput by orders of magnitude, increasing the range of RNAs that can be identified, and coupling this technique with other approaches to single-cell 'omics. In parallel, the Moffitt laboratory is leveraging this technique to ask fundamental questions at the microbiome-host interface in mammals--questions such as how the microbiome is spatially organized, how does this organization affect interactions within this community and with the host, and how are gut cell types remodeled in response to perturbations to the microbiome?
Spatial transcriptomics techniques, such as MERFISH, were named Nature Methods technique of the Year in 2020, and these techniques are seeing rapid adoption in both academic and industrial research. Individuals that hold this position will be integrated into multiple ongoing studies that are both leveraging MERFISH and extending this technique; thus, the position offers the opportunity to be trained in this emerging and exciting new technology.
The position is for one year with the possibility of extension. For more information on our research interests see: https://moffittlab.github.io/
Under immediate supervision, performs a variety of routine and specialized laboratory experimental, specimen preparations and experimental procedures for the purpose of research. Assists in the use of specialized laboratory equipment and analysis of data generated by this equipment
Image analysis on high-performance computing clusters
Standard molecular biology protocols, including qPCR, reverse transcription, and PAGE
The knowledge of theories, principles, and concepts acquired through completion of a Bachelor's degree in biology, chemistry, physics, or engineering.
Highly motivated, organized, and capable of working in a team.
No prior experience with single-molecule RNA imaging, high-performance computing, or programming languages such as Python and Matlab is required but would be useful.
During a public health emergency, individuals in this role may be expected to take on additional duties to respond to organizational needs.
BCH offers competitive compensation and benefits
Boston Children's Hospital is an Equal Opportunity / Affirmative Action Employer. Qualified applicants will receive consideration for employment without regard to their race, color, religion, national origin, sex, sexual orientation, gender identity, protected veteran status or disability.
Internal Number: 3147356
About Boston Children's Hospital
Boston Children's Hospital is a 395-bed comprehensive center for pediatric health care. As one of the largest pediatric medical centers in the United States, Children's offers a complete range of health care services for children from birth through 21 years of age.