As a UW employee, you have a unique opportunity to change lives on our campuses, in our state and around the world. UW employees offer their boundless energy, creative problem solving skills and dedication to build stronger minds and a healthier world.
UW faculty and staff also enjoy outstanding benefits, professional growth opportunities and unique resources in an environment noted for diversity, intellectual excitement, artistic pursuits and natural beauty.
The Division of Cardiology is a large, complex Division in the Department of Medicine with over 100 faculty, 31 fellows and over 100 professional, classified and hourly employees spread across 6 campuses: UW-Montlake, Harborview Medical Center, UW-Northwest, Edmonds Cardiology Clinic, VA Puget Sound Hospital and UW South Lake Union.
The Division of Cardiology has an outstanding opportunity for a full-time (1.0 FTE) Research Scientist/Engineer 3 .
Elucidate the role of smooth muscle transforming growth factor beta signaling in aortic disease.
Job Duties and Responsibilities
After contributing to designing and planning, lead the execution of experiments that investigate the role of transforming growth factor beta signaling in smooth muscle cells in the development of aortic aneurysms. Experiments will involve generation of mice with multiple transgenes.
Help design breeding schemes, plan experimental manipulations of the mice, time the harvests, and select methods for harvesting and processing tissues. The mice will be harvested and analyzed according to protocols that are either developed by the employee or adapted by the employee from existing laboratory protocols.
Analytic methods will include tissue microdissection, tissue preparation for histologic analysis or single-cell analysis (e.g., by flow cytometry), fluorescence microscopy, laser-capture microdissection, bulk RNA sequencing, single-cell RNA sequencing or other approaches identified by the team.
Help develop in vitro experimental systems to test hypotheses that emerge from the in vivo studies. In vitro techniques may include co-immunoprecipitation, immunoblotting, as well as flow cytometry and mass spectrometry (performed by others in specialized core facilities).
Interact productively with collaborating scientists who have complementary expertise.
Act as the lead scientist in the laboratory in the context of these collaborations and interact with collaborators largely independently of the Principal Investigator.
Help supervise students in the laboratory.
Perform statistical analysis of experimental data, using computer software.
Prepare publication-quality figures and tables for manuscripts and presentations; help draft text for manuscripts and grant applications.
Contribute to laboratory management tasks, along with others in the group.
As a UW employee, you will enjoy generous benefits and work/life programs. For a complete description of our benefits for this position, please visit our website, click here.
Bachelors Degree in Biological Science plus a minimum of 3 years' work experience
Equivalent education/experience will substitute for all minimum qualifications except when there are legal requirements, such as a license/certification/registration.
Excellent communication skills (both verbal and written)
Specific knowledge, skills and abilities required to perform the job satisfactorily include: Knowledge of molecular biology and biochemistry techniques including protein and nucleic acid extraction and analysis. Ability to identify proper experimental controls and include them in experiments. Ability to interact productively with colleagues in other disciplines. Ability to take initiative to read relevant literature and suggest new experimental approaches as well as modifications of existing approaches. Ability to train and supervise students. Meticulous record keeping, including laboratory notebook maintenance and data entry into computerized data bases. Ability to prepare drafts of figures and tables for oral presentations and manuscripts and to present research in an organized and critical manner.
Experience generating complex transgenic mice and analyzing their phenotypes with techniques including flow cytometry, immunofluorescence, histology, and both protein and RNA analyses. Familiar with basic bioinformatics tools such as those used to identify differentially expressed genes or perform pathway analyses. Experience performing protein analyses in cultured cells including immunoblotting. Knowledge of transforming growth factor beta signaling pathway and experience measuring it in tissues and cultured cells. Experience with wire myography. Ability to write drafts of portions of manuscripts and grant applications. Ability and willingness to provide constructive input on other projects in the laboratory.
CONDITIONS OF EMPLOYMENT
Application Process: The application process for UW positions may include completion of a variety of online assessments to obtain additional information that will be used in the evaluation process. These assessments may include Workforce Authorization, Cover Letter and/or others. Any assessments that you need to complete will appear on your screen as soon as you select “Apply to this position”. Once you begin an assessment, it must be completed at that time; if you do not complete the assessment you will be prompted to do so the next time you access your “My Jobs” page. If you select to take it later, it will appear on your "My Jobs" page to take when you are ready. Please note that your application will not be reviewed, and you will not be considered for this position until all required assessments have been completed.
Founded in 1861, the University of Washington is one of the oldest public institutions in the west coast and one of the preeminent research universities in the world. The University of Washington is a multi-campus university comprised of three different campuses: Seattle, Tacoma, and Bothell. The Seattle campus is made up of sixteen schools and colleges that serve students ranging from an undergraduate level to a doctoral level. The university is home to world-class libraries, arts, music, drama, and sports, as well as the highest quality medical care in Washington State and a world-class academic medical center. The teaching and research of the University’s many professional schools provide undergraduate and graduate students the education necessary toward achieving an excellence that will serve the state, the region, and the nation. As part of a large and diverse community, the University of Washington serves more students than any other institution in the Northwest.