Under the direct supervision of senior research staff in the Melton Lab, the Research Assistant will support experiments focused on characterizing beta cells derived from in vitro directed differentiation of iPS and/or genetically modified cell lines.
The Research Assistant (RA) will be part of a team of 2-4 research staff, which together will be responsible to produce human islet cells and ensure the generation of highest quality material for research purposes. The RA will be responsible with daily cultures of human pluripotent stem cells including maintenance, expansion and differentiation to various lineages. The RA will perform quantitative and qualitative assessments of beta cell directed differentiation using assays that include immunohistochemistry, FACS, gene expression, DNA and RNA sequencing, and physiological tests for beta cell function such as glucose tolerance testing (GTT), glucose stimulated insulin secretion (GSIS), ELISAs. Research assistants are expected to co-develop standard protocols and quality control measures, and independently collect and record experimental data and update written protocols and methods based on findings. Other job duties may include laboratory maintenance (including cleaning equipment), monitoring/ordering supplies, and instruct staff and students in derivation and differentiation methods.
Two years related work experience in the expansion and differentiation of ES/iPS cell lines. This position requires a strong commitment and the willingness to work irregular hours, some holiday and weekend coverage will be required. This is a grant-funded position with a 2-year commitment preferred and the possibility of extension based on satisfactory performance and continued availability of funding.
Salary Grade: 052
Union: 55 - Hvd Union Cler & Tech Workers
College background or equivalent work experience in Biology or related field.
Bachelor's degree in biology or closely related field is required as is related work experience in a biological research laboratory. Experience with culturing human pluripotent stem cells including maintenance, expansion and differentiation to various lineages is preferred. Ability to work in a collaborative, team-oriented environment, and strong communication skills are required.
EQUAL OPPORTUNITY EMPLOYER: We are an equal opportunity employer and all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability status, protected veteran status, or any other characteristic protected by law.
Internal Number: 53955BR
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