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POSITION PURPOSE This is an exciting opportunity for a Research Scientist/Engineer 2 (RSE2) to work with new long-read DNA and RNA sequencing technology. Projects will include using long-read sequencing technology to investigate the role of genomic structural variation in human disease, performing DNA and RNA sequencing of individuals with unsolved genetic disorders, and collaborating with a diverse group of investigators to design and implement long-read sequencing projects. The position will be actively involved in development of long-read sequencing projects both within the laboratory and with outside collaborators.
POSITION SUMMARY The Department of Genome Sciences (GS) is part of the University of Washington School of Medicine and comprises 30 labs addressing leading edge questions in biology and medicine by developing and applying genetic, genomic, and computational approaches taking advantage of genomic information now available for a host of species. GS is making major contributions in the application of genome science to understand the fundamentals of biology and to improve human health and wellbeing. The department has gone through significant expansion since its beginning in 2001 with annual grant awards of $57M for FY20. The department has 62 training, teaching, and research faculty.
The position will be supported by the Brotman Baty Institute for Precision Medicine (BBI), a joint venture between the University of Washington, the Fred Hutchinson Cancer Research Center and Seattle Children’s Hospital, made possible by a $50 million gift from the Brotman and Baty Families. The BBI offers a dynamic work environment with cutting-edge science and computational resources. The RSE2 will actively contribute to ongoing laboratory research projects and work with local and international collaborators to design and implement long-read sequencing projects on the Oxford Nanopore platform. Work collaboratively with other technicians; assist graduate students, postdoctoral fellows, and clinical fellows with ongoing research projects. Independently perform scientific investigative procedures under limited supervision.
RESPONSIBILITIES (100% research related duties)
Perform sequencing projects on a range of Oxford Nanopore platforms (Flongle, MinION, GridION, PromethION). Includes library preparation, sequencer operation, data quality monitoring, and basic data analysis. Full hands-on training provided, if necessary.
DNA and RNA isolation from various sources.
Validation of findings using PCR and qPCR.
Sample tracking and database maintenance.
Perform routine analysis of data using the command line: assist with troubleshooting and daily quality control of data and assist researchers with data analysis, organization and interpretation, and experimental design.
Perform related duties as required.
Bachelor’s degree in Biology, Biochemistry, Microbiology or related Life Sciences field and two or more years related experience in a molecular biology, genomics, or biology laboratory.
Some exposure to next-generation sequencing and genomics laboratory techniques.
Able to work independently and as part of a multidisciplinary team.
Able to learn new techniques.
Attention to detail and strong organizational abilities.
Must have basic computing skills.
Master’s degree in Microbiology, Genetics or related Life Sciences field.
Experience and knowledge of the fundamental concepts, practices and procedures required for working in a genomics laboratory.
Knowledge of next-generation sequencing platforms and techniques.
PCR & qPCR for genotyping.
DNA & RNA isolation.
Some knowledge of sequence analysis, programs, and programming for bioinformatics.
Familiarity with human genomics terms and concepts.
Experience with UNIX/*NIX command line, BASH shell scripting, and Python.
Application Process: The application process for UW positions may include completion of a variety of online assessments to obtain additional information that will be used in the evaluation process. These assessments may include Work Authorization, Cover Letter and/or others. Any assessments that you need to complete will appear on your screen as soon as you select “Apply to this position”. Once you begin an assessment, it must be completed at that time; if you do not complete the assessment you will be prompted to do so the next time you access your “My Jobs” page. If you select to take it later, it will appear on your "My Jobs" page to take when you are ready. Please note that your application will not be reviewed, and you will not be considered for this position until all required assessments have been completed.
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