Research Technician - Weinstock Lab

We are seeking a highly motivated candidate to fill a full-time research technician position in Dr. David Weinstock’s laboratory at the Dana-Farber Cancer Institute. We are a translational leukemia and lymphoma research laboratory that uses a variety of biochemical, molecular and computational approaches to identify new therapeutic targets, develop preclinical models of disease and test novel therapeutics both in vitro and in vivo. The research technician would work on a project studying regulatory circuits that underlie therapeutic resistance and identification of biomarkers for building computational models predicting response to therapy in T cell lymphomas. The successful candidate will be a team player capable of handling multiple tasks and adaptable to a fast-paced research environment. The candidate will benefit from working in a vibrant collaborative environment (physicians, biologist, technologist and computationalist) and will receive training in omics methodologies and state-of-the-art spatial methodologies for studying the cell-cell relationships within the tumor microenvironment.

Located in Boston and the surrounding communities, Dana-Farber Cancer Institute brings together world renowned clinicians, innovative researchers and dedicated professionals, allies in the common mission of conquering cancer, HIV/AIDS and related diseases. Combining extremely talented people with the best technologies in a genuinely positive environment, we provide compassionate and comprehensive care to patients of all ages; we conduct research that advances treatment; we educate tomorrow's physician/researchers; we reach out to underserved members of our community; and we work with amazing partners, including other Harvard Medical School-affiliated hospitals.

The technician will be responsible for conducting experiments and performing data analysis to advance the research progress in the lab. As part of this work, they will be responsible for the following:

• Data analysis using R/ Python;
• Analyzing RNA-Seq and DNA-Seq data to detect differentially expression and gene mutations;
• Image based single-cell analysis preferred, but not a requirement and on the job, training can be provided;
• Immunofluorescent Staining of Paraffin-Embedded Tissue;
• Maintenance of cell lines and primary cells in culture;
• Basic handling of DNA and RNA including sample isolation, PCR and preparation of samples for sequencing;
• Protein isolation, Western blotting, and immunoprecipitation experiments;
• Preparation of reagents and media used in lab experiments;
• Maintaining detailed records of their laboratory work, results and data analyses; and
• Sharing responsibilities for operation and maintenance of standard lab equipment.

The technician should be able to work independently, but also be open and receptive to suggestions and feedback from the team/project leader. Attention to detail in completing tasks and a strong work ethic is essential. This includes willingness to critically read and keep up to date on the latest scientific literature, logically troubleshoot problems, offer both intellectual and technical input during collaborative meetings and assist in the preparation of presentations. The technician should have flexibility in their schedule to work some evenings and weekends as needed to meet project goals. We seek candidates who have at least 2 years of availability. The job would provide great exposure to someone who is considering going to graduate school or medical school in the future. 

• Minimum of a bachelor’s degree in bioinformatics, computational biology, biology or molecular biology is required.
• If you are a computational biologist but would like to work in a wet-lab environment or a wet-lab researcher with knowledge in Python/R, you are encouraged to apply. We can train you in the other aspects of work.
• Excellent communication skills and ability to synthesize research findings for presentation in both oral and written formats.

Dana-Farber Cancer Institute is an equal opportunity employer and affirms the right of every qualified applicant to receive consideration for employment without regard to race, color, religion, sex, gender identity or expression, national origin, sexual orientation, genetic information, disability, age, ancestry, military service, protected veteran status, or other groups as protected by law.