Proficient research laboratory specialist with a sophisticated understanding of specific, advanced techniques and a solid foundation in the underlying science, gained from professional experience. Works independently and manages daily research projects.
The Research Specialist participates in planning experiments and evaluating/interpreting test results utilizing detailed knowledge of research laboratory procedures and practices. Capable of independent judgment in the selection, development, performance and evaluation of research methods; operates and maintains appropriate equipment.
Specific Duties & Responsibilities
Performs cell culture and processes biofluids and tissues from human donors and animal models. Performs fast protein liquid chromatography (FPLC)-based size separation to obtain proteins and protein complexes, confirming identity of selected isolated proteins. Isolates RNA, performs quality control, and prepares RNA sequencing libraries using methods including advanced, ligation-free preparations. Separation and characterization of extracellular vesicles. Assists with analysis and graphical representation of RNA-seq data. Troubleshoots and optimizes techniques based on scientific knowledge and research experience. Liases with bioinformatics collaborators.
Follows established procedures or protocols and utilizes aseptic and RNase-free technique to perform laboratory tests and/or experiments which include: conventional and real-time PCR, electrophoretic separations (agarose and acrylamide gels), Western blot, ELISA, cloning and/or subcloning, extracellular vesicle isolation and characterization, electron microscopy, nanoparticle tracking analysis, resistive pulse sensing, flow cytometry, and other molecular and cellular biology techniques.
Learn and train in additional procedures in order to expand skills, foster individual growth, and directly contribute to and facilitate laboratory success.
Uses various software applications, such as spreadsheets, relational databases, electronic mail, World Wide Web, statistical packages, and graphics packages to assemble, manipulate and/or format data and/or test reports.
Participates in the organization and operation of the laboratory, including oversight of undergraduate student researchers. Performs general lab maintenance duties and maintains excellent written records and electronic databases.
Uses universal safety precautions to protect self and co-workers from biohazardous materials, including blood-borne pathogens. Completes annual university biohazard/universal precaution/radiation safety training, as appropriate. Monitors biohazard/radiation safety standards and proper handling of potentially hazardous chemical and biological agents and/or radiation sources in the workplace.
Bachelor's degree, or equivalent, in biology, chemistry or related field plus 1 year of laboratory experience performing relevant techniques.
Master's degree, with related graduate research, may substitute for experience to the extent permitted by the JHU equivalency formula:
* JHU Equivalency Formula: 30 undergraduate degree credits (semester hours) or 18 graduate degree credits may substitute for one year of experience. For jobs where equivalency is permitted, up to two years of non-related college course work may be applied towards the total minimum education/experience required for the respective job. *
Special Knowledge, Skills, and Abilities
Work produced is subject to precise measures of quantity and quality Near vision to see objects clearly within 20 inches Sharp focus to adjust vision when doing close work that changes in distance from eyes Full spectrum vision to identify and distinguish color Finger dexterity required to manipulate objects with fingers rather than with whole hand(s) or arm(s), for example, using a keyboard Handling by seizing, holding, grasping, turning or otherwise working with the hand or hands, but without finger dexterity Sitting in a normal seated position for extended periods of time Occasionally lifting, carrying objects weighing 10 lbs. or less Occasionally pushing, pulling objects weighing 30 lbs. or less Ability to move about
Classified Title: Research Specialist Working Title: Research Specialist Role/Level/Range: ACRP/03/MA Starting Salary Range: $16.13 - $22.16; Commensurate with Experience Employee group: Full Time Schedule: Monday-Friday, 8:30 am - 5:00 pm, Up to 37.5 hrs/ week Exempt Status: Non-Exempt Location: 04-MD:School of Medicine Campus Department name: 10002778-SOM Comp Med Retrovirus Bio Laboratory Personnel area: School of Medicine
The successful candidate(s) for this position will be subject to a pre-employment background check.
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The following additional provisions may apply depending on which campus you will work. Your recruiter will advise accordingly.
During the Influenza ("the flu") season, as a condition of employment, The Johns Hopkins Institutions require all employees who provide ongoing services to patients or work in patient care or clinical care areas to have an annual influenza vaccination or possess an approved medical or religious exception. Failure to meet this requirement may result in termination of employment.
The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.
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Johns Hopkins University remains committed to its founding principle, that education for all students should be grounded in exploration and discovery. Hopkins students are challenged not just to learn but also to advance learning itself. Critical thinking, problem solving, creativity, and entrepreneurship are all encouraged and nourished in this unique educational environment. After more than 130 years, Johns Hopkins remains a world leader in both teaching and research. Faculty members and their research colleagues at the university's Applied Physics Laboratory have each year since 1979 won Johns Hopkins more federal research and development funding than any other university. The university has nine academic divisions and campuses throughout the Baltimore-Washington area. The Krieger School of Arts and Sciences, the Whiting School of Engineering, the School of Education and the Carey Business School are based at the Homewood campus in northern Baltimore. The schools of Medicine, Public Health, and Nursing share a campus in east Baltimore with The Johns Hopkins Hospital. The Peabody Institute, a leading professional school of music, is located on Mount Vernon Place in downtown Bal...timore. The Paul H. Nitze School of Advanced International Studies is located in Washington's Dupont Circle area.