Lawrence Berkeley National Lab's (LBNL) Agile BioFoundry (ABF) and Joint BioEnergy Institute (JBEI) programs in the Biological Systems & Engineering Division has an opening for a Research Scientist with experience in analytical mass spectrometry, proteomics methods development, and automation. In this exciting role, you will independently perform the development and optimization of bioanalytical methods for protein quantification in microbes of interest to the ABF and JBEI. You will (a) initiate and direct methods development for high-throughput, quantitative proteome analysis and guide implementation at other ABF labs and across JBEI, (b) lead efforts to automate omics protocols to enable high-throughput sample preparation and data acquisition methods (c) apply methods to assess the effects of genetic manipulation of microbes on the production of bioproducts by using chromatographic and omics techniques, and (d) contribute to overall ABF and JBEI project development and data analysis methods to inform subsequent process improvements. What You Will Do: - Lead design and implementation of strategies for complex proteomic assays of engineered microbes (e.g., shotgun proteomics for discovery, identification of post-translational modifications, targeted proteomic methods for validation and quantification).
- Contribute to ABF project goals by rapidly characterizing engineered microbe performance (e.g., LC/MS proteomic assessment of gene expression and contribute to the measurement of metabolites via LC/MS and HPLC-UV).
- Develop automated sample preparation assays and lead the transfer to other ABF labs and across JBEI.
- Develop high-throughput LC-MS/MS assays to evaluate changes to microbial proteome composition under a wide variety of lab and industrial conditions.
- Contribute to efforts to support data reproducibility and FAIR data sharing of proteomic data in the ABF and JBEI.
- Collaborate with molecular biologists, analytical, and computational teams across the ABF, JBEI, and at the Lab, to develop quantitative metabolic and stress response models that impact production levels and production of engineered microbes.
- Participate in group meetings, project meetings, and seminars.
- Lead the development and authorship of peer-reviewed publications.
- Perform research lab procedures efficiently and with attention to safety and necessary quality control procedures.
- Maintain an accurate and detailed scientific logbook of all experiments and protocols performed. Ensure that others could duplicate results.
Additional Responsibilities as needed: - Experience in the examination of engineered microbial strains in a range of cultivation modes and regimes - to collect growth and production data, and samples for multi-omics experiments (RNA-seq, resequencing, protein, and metabolite analysis).
What is Required: - Five years of relevant experience in biotechnology applications that use microbiology, molecular biology, systems biology research and development in microbial systems beyond the highest degree in a related field.
- Demonstrated experience performing laboratory research using standard techniques of analytical chemistry and molecular biology protocols, including extraction and manipulation of proteins from microbes.
- Extensive experience with proteomic sample preparation, data acquisition software (e.g., MassHunter, Skyline), and data analysis tools (e.g., Mascot, Scaffold, R, jupyter notebooks).
- Extensive experience using and maintaining liquid chromatography/mass spectrometric systems (e.g., QQQ, QTOF, Orbitrap LC/MS).
- Experience with automated liquid handling systems (e.g., Biomek, Hamilton, Labcyte).
- Experience with working in collaboration with user-facilities and other computational/analytical/biological teams.
- Strong publication record.
- Effective interpersonal, verbal, and written communication skills, including public speaking and presentation skills.
- Ability to work with guidance from a principal investigator and collaborators as well as to conduct experiments independently, maintain appropriate records, and manage data.
- Ability to work well independently as well as a part of a diverse team and effectively interact with colleagues with a broad range of skills.
- Ability to troubleshoot problems, recommend actions and serve as work lead for research assistants and research associates.
- Advanced analytical, organizational, and record-keeping skills.
Desired Qualifications: - Ph.D. in Analytical Chemistry, Biochemistry, or a related field.
- Experience with metabolomic methods development and sample preparation.
- Experience in culturing bacteria with liquid and solid media.
For full consideration, please apply by March 11, 2021. Notes: - This is a full-time career appointment, exempt (monthly paid) from overtime pay.
- This position may be subject to a background check. Any convictions will be evaluated to determine if they directly relate to the responsibilities and requirements of the position. Having a conviction history will not automatically disqualify an applicant from being considered for employment.
- Work will be primarily performed at EmeryStation East -- Bldg. 978, 5885 Hollis St., 4th floor, Emeryville, CA.
Learn about us! Biological Systems and Engineering Division Vision: To lead efforts that combine the power of biology with the tools of engineering to develop sustainable energy and biomanufacturing solutions and improve human health. Mission: Advance a mechanistic and predictive understanding of complex biological systems over multiple scales in terms of their responses to manipulation, stress, disease and environmental challenges and translate this knowledge using engineering principles to develop resilient systems, tools, and processes for the efficient production of fuels, chemicals, materials, tissues, and therapeutics. Berkeley Lab Berkeley Lab (LBNL) addresses the world's most urgent scientific challenges by advancing sustainable energy, protecting human health, creating new materials, and revealing the origin and fate of the universe. Founded in 1931, Berkeley Lab's scientific expertise has been recognized with 13 Nobel prizes. The University of California manages Berkeley Lab for the U.S. Department of Energy's Office of Science. Working at Berkeley Lab has many rewards including a competitive compensation program, excellent health and welfare programs, a retirement program that is second to none, and outstanding development opportunities. To view information about the many rewards that are offered at Berkeley Lab- Click Here. Equal Employment Opportunity: Berkeley Lab is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, or protected veteran status. Berkeley Lab is in compliance with the Pay Transparency Nondiscrimination Provision under 41 CFR 60-1.4. Click here to view the poster and supplement: "Equal Employment Opportunity is the Law. Lawrence Berkeley National Laboratory encourages applications from women, minorities, veterans, and other underrepresented groups presently considering scientific research careers. |
