An entry-level, full time Research Assistant position is available in the laboratory of Dr. Karthickeyan Chella Krishnan in the Department of Pharmacology and Systems Physiology at the University of Cincinnati College of Medicine.
Dr. Chella Krishnan’s laboratory is focused on understanding how genetics and sex differences influence the mitochondrial (dys)function and increases the susceptibility to cardiovascular and metabolic diseases. The K lab uses a population-based ‘systems genetics’ approach to integrate information on natural genetic variations with molecular phenotypes (such as gene expression, proteomics, etc.) and clinical phenotypes, to understand the effects of genetics and sex differences on obesity, heart failure, and non-alcoholic fatty liver disease.
The successful applicant will assist in the designing, performing, and analyzing of studies that investigate the effects of genetics, diet and sex on cardio-metabolic diseases as well as be responsible for general lab maintenance and the maintenance of the mouse colony, including a transgenic mouse breeding colony.
Performs common molecular and cell biology approaches including mammalian cell tissue culture, DNA, RNA, and protein isolation, western blotting, immunocytochemistry, bacterial transformation, plasmid DNA preparation, PCR, cell transfection, DNA subcloning, and manipulation.
Performs mitochondrial isolation and extracellular flux analysis of bioenergetic function.
Assist in the design and execution of animal studies (including mouse dissection and tissue collection).
Perform general lab maintenance duties including, but not limited to, meticulous preparation of reagents, managing protocols, ordering of supplies and tracking of inventory.
Perform experiments and records data according to research protocols.
Interpret data and assist in judging validity of data.
May perform statistical and graphical analysis of data.
May perform surgical procedures in mice.
Five (5) years of relevant work experience and/or other specialized training can be used in lieu of education requirement.
Additional Qualifications Considered
One year of work experience.
Excellent work ethic, strong communication skills, honesty, and ability to work well with minimum supervision or as a part of a group.
Experience in molecular biology, cell biology, and tissue culture techniques.
Experience in working with mice in research.
Experience using basic laboratory equipment (centrifuges, CO2 incubators, spectrophotometer, PCR machines, pH meter, balances, plate reader, light microscopes, and gel documentation).
Familiar with both PC and Apple computer systems and software programs (MS Word, Excel, and PowerPoint).
Physical Requirements/Work Environment
Sitting - Continuously
Repetitive hand motion (such as typing) - Often
Hearing, listening - Often
Talking - Often
Standing - Often
Walking - Often
Bending - Often
Stooping - Often
Lifting - up to 20 pounds - Often
Kneeling, squatting - Seldom
Noise: There is sufficient noise to cause you to shout in order to be heard above the noise level.
Hazards: Includes a variety of physical conditions, such as proximity to moving mechanical parts of machinery or tools used to perform work, moving vehicles, electrical current, working on scaffolding and high places, or exposure to chemicals.
Atmospheric Conditions: Conditions that affect the respiratory system, such as fumes, odors, dusts, mists, gases, or poor ventilation.
The University of Cincinnati, as a multi-national and culturally diverse university, is committed to providing an inclusive, equitable and diverse place of learning and employment. As part of a complete job application you will be asked to include a Contribution to Diversity and Inclusion statement.
As a UC employee, and an employee of an Ohio public institution, if hired you will not contribute to the federal Social Security system, other than contributions to Medicare. Instead, UC employees have the option to contribute to a state retirement plan (OPERS, STRS) or an alternative retirement plan (ARP).
The University of Cincinnati is an Affirmative Action / Equal Opportunity Employer / Minority / Female / Disability / Veteran.
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Internal Number: 60162-en_US
About University of Cincinnati
HISTORY IN BRIEF - The University of Cincinnati is a public urban serving research institution that was founded in 1819 and today is one of the country’s largest universities offering more than 350 academic programs to more than 44,000 students. UC has many top ranked programs in areas such as Music and Arts, Medical and Human-Services, Criminal Justice and more. Located in an urban setting, UC is close to the heart of Cincinnati and allows easy access for students to enjoy all that the city offers. UC is also the alma mater of many notables such as President William Howard Taft; Albert Sabin, developer of the oral polio vaccine; Astronaut Neil Armstrong and Mary Weinberg, 2008 Olympic gold medalist. UC is classified as a Research University (Very High Research Activity) by the Carnegie Commission and is ranked as one of America’s top 35 public research universities by the National Science Foundation. UC jumped 17 spots in the U.S. News & World Report rankings in the past two years alone. In addition to being named a “green university” by Princeton Review, UC has been named one of the world’s most beautiful campuses by Forbes and Delta Sky magazines. Learn more at www.uc.edu.MISSIO...N STATEMENT - The University of Cincinnati serves the people of Ohio, the nation, and the world as a premier, public, urban research university dedicated to undergraduate, graduate, and professional education, experience-based learning, and research. We are committed to excellence and diversity in our students, faculty, staff, and all of our activities. We provide an inclusive environment where innovation and freedom of intellectual inquiry flourish. Through scholarship, service, partnerships, and leadership, we create opportunity, develop educated and engaged citizens, enhance the economy and enrich our University, city, state and global community.